Investigations of the Mechanism of Vanadium Haloperoxidases

该奖项由无机化学、生物无机化学和有机金属化学项目颁发，支持加州大学圣巴巴拉分校化学和生物化学系的艾莉森·巴特勒的研究。巴特勒正在研究卤代过氧化物酶，更具体地说是在几种类型的海藻中发现的钒溴过氧化物酶。这些酶的作用机制以及海洋天然产物中卤代萜类和乙酸基的生物发生机制将被探索。利用定点突变，巴特勒将确定选定的氨基酸残基在酶中的功能作用，并准备适合体外反应的突变体。这些突变体最终可能用于工业合成适合医学应用的卤代化合物。除了所提出的基础生物无机化学和酶学之外，这项工作对海洋生物学和药物化学具有广泛的意义。巴特勒还在新兴的跨学科领域培训了一批不同的学生

This award by the Inorganic, Bioinorganic and Organometallic Chemistry program supports research by Alison Butler, University of California Santa Barbara Department of Chemistry and Biochemistry. Butler is studying haloperoxidase enzymes, more specifically the vanadium bromoperoxidases found in several types of marine algae. The mechanisms of these enzymes and the biogenesis of halogenated terpene and acetogenin marine natural products will be explored. Using site-directed mutagenesis, Butler will determine the functional role of selected amino acid residues in the enzymes and prepare mutants suitable for in vitro reactivity. These mutants may eventually be used for the industrial synthesis of halogenated compounds suitable for medical applications.In addition to the fundamental bioinorganic chemistry and enzymology proposed, the work has broad implications for marine biology and pharmaceutical chemistry. Butler also trains a diverse group of students in an emerging interdisciplinary field