Wechselwirkungen von polyphilen Molekülen mit Lipidmonolayern und Lipidvesikeln

本项目的目标是研究和阐明水溶液中低分子量和聚合的亲水性分子与脂质模型膜和脂质单层的相互作用。低分子量亲聚物将具有T-和X-样结构，并且将在C组中合成。Treßerske（TP 2），而聚合的亲聚物将从Kreßler（TP 3）组获得。首先将对溶致行为及其在水中的聚集进行研究。因此，侧链的化学结构以及嵌段序列的影响将被研究，随后通过调查的化合物在空气-水界面的表面活性。脂质模型膜和脂质单层将作为模板，用于亲聚物的结合和在界面处形成结构。结合强度是由于与膜的疏水和亲水相互作用，并且将通过改变亲聚物的化学结构而变化。用于研究聚集行为的基本方法是光散射法、透射电子显微镜、用于测定临界胶束浓度（cmc）的等温滴定量热法（ITC）以及单层方法（落射荧光光谱法、红外反射吸收光谱法（IRRAS）、布鲁斯特角显微镜法）。将以平行的方式进行亲聚物与脂质双层和脂质单层的相互作用的研究。低分子量亲聚物的研究目的是通过改变侧链结构来改变这些分子的相互作用以及膜中或膜处的所得结构形成和分离。对于高分子亲多性体，高分子和手性脂质之间可能的手性识别起着重要的附加作用。对于这些研究，将使用热方法（如ITC和DSC）以及光谱方法（如固体支撑膜上的FT-IR-光谱法（FT-IR-ATR）和脂质单层上的FT-IRRAS）。与巴西亚组（TP 6）合作，将对GUV进行CLSM研究，并结合染料流出量测量。还将进行使用小囊泡的类似染料释放实验，以阐明在掺入亲聚物后膜的渗透屏障的完整性。在与Saalwächter（TP 5）小组的密切合作下，将使用相同的系统进行额外的NMR实验。

Goals of this project are investigations and elucidation of the interactions of low molecular weight and polymeric polyphilic molecules in aqueous solution with lipid model membranes and lipid monolayers. Low molecular weight polyphiles will have T- and X-like structures and will be synthesized in the group of C. Tschierske (TP 2) while the polymeric polyphiles will be obtained from the group Kreßler (TP 3). Investigations on the lyotropic behaviour and their aggregation in water will be carried out first. Thereby influence of the chemical structure of the side chains as well as the block sequence will be studied followed by investigations of the surface activity of the compounds at the air-water interface. Lipid model membranes and lipid monolayers will serve as templates for the binding of the polyphiles and resulting structure formation at the interface. The binding strength is due to hydrophobic and hydrophilic interactions with the membranes and will be varied by changing the chemical structure of the polyphiles. The basic methods for studying the aggregation behaviour are light scattering methods, transmission electron microscopy, isothermal titration calorimetry (ITC) for the determination of the critical micellar concentration (cmc), as well as monolayer methods (epi-fluorescence-spectroscopy, infrared-reflection-absorptionspectroscopy (IRRAS) Brewster-angle-microscopy). Studies on the interaction of polyphiles with lipid bilayers and lipid monolayers will be carried out in a parallel fashion. Goal of studies with the low molecular weight polyphiles is to modify the interactions of these molecules and the resulting structure formation and segregation in or at the membranes by changes in the lateral chain structure. For polymeric polyphiles possible chiral recognition between polymers and chiral lipids plays an important addional role. For these studies thermal methods such as ITC and DSC will be used as well as spectroscopic methods such as FT-IR-Spectroscopy on solid supported membranes (FT-IR-ATR) and FT-IRRAS on lipid monolayers. In cooperation with the group Bacia (TP 6) CLSM studies on GUVs will be performed coupled with dye efflux measurements. Similar dye release experiments with small vesicles will also be carried out to elucidate the integrity of the permeation barrier of the membrane after incorporation of polyphiles. In close cooperation with the group Saalwächter (TP 5) additional NMR-experiments will be carried out with the same systems.