SGER: BAC end Sequencing to Quality Control BAC Libraries

细菌人工染色体（BAC）克隆由于其大的插入长度和稳定性，已成为基因组作图、DNA测序以及比较和功能基因组学项目的重要组成部分，以回答进化、农业和生物医学问题。因此，美国国家科学基金会（NSF）启动了一项计划，资助31个动物BAC库和32个植物BAC库的建设，这些库来自跨越生命系统发育树的物种。显然，这些库应该是高质量的，这样才最有用。因此，质量控制成为一项基本要求。BAC端测序提供了几个关于BAC文库的关键信息，包括污染、随机性和插入大小。在这里，我们建议从每个文库中随机选取约100个克隆进行测序，并进行序列分析，以评估在NSF项目下正在建设的63个BAC文库的质量。

0306190ZhaoDue to their large insert size and stability, Bacterial Artificial Chromosome (BAC) clones have emerged as an essential component in genome mapping, DNA sequencing, as well as comparative and functional genomics projects to answer evolutionary, agricultural and biomedical questions. As such, the National Science Foundation (NSF) has initiated a program that funds the construction of 31 animal BAC libraries and 32 plant BAC libraries from species spanning the phylogenetic tree of life. Clearly, these libraries should be of high quality to be most useful. Quality control therefore becomes an essential requirement. BAC end sequencing provides several pieces of critical information about a BAC library regarding contaminations, randomness and insert size. Here we propose to end sequence approximately 100 random clones from each library and conduct sequence analyses to assess the quality of the 63 BAC libraries that are being constructed under the NSF program.