MRI: Development of Fluorescence-Based Spectroscopy and Imaging Microfluidics System for Surface Chemical and Geometric Optimization

ABSTRACTProposal没有。首席研究员：J. Schneider，卡内基梅隆大学。该资助用于开发一种基于荧光的仪器，用于微流体系统中流动和吸附的可视化。该仪器由倒置荧光显微镜、固态激光器、高速CCD相机、低噪声直流电源和一些较小的设备组成。吸附量的测量将由全内反射荧光（TIRF）完成。微流控装置将使用标准软光刻方法构建，允许控制微通道几何形状和表面化学。流体流动可以通过电渗透或注射泵来诱导，这取决于应用。该设备将支持三个具有代表性的研究项目：首先是评估横向扩散对DNA杂交动力学的贡献，以表面结合探针作为提高生物传感器性能的手段。第二是开发一种微尺度色谱系统，利用DNA结合表面活性剂从复杂混合物中分离目标DNA。第三是改进芯片实验室系统计算机辅助设计的数值算法的开发和验证。仪器的影响将在许多方面。这可能是第一个包含在线TIRF的微流体可视化仪器，为上述研究提供至关重要的表面吸附信息。此外，校园内涉及微流体和MEMS的许多小组将把该仪器作为共享资源。我们还将进行一系列微流体实验（可作为基于网络的资源），作为运输课程的示例和问题集。我们还将为参加卡内基梅隆大学少数民族学生暑期学院的高中生开发实践项目。这将作为一个令人兴奋的领域的介绍，并使学生熟悉最先进的实验室设备。

ABSTRACTProposal No. CTS-0320548Principal Investigator: J. Schneider, Carnegie Mellon UniversityThis grant is to develop a fluorescence-based instrument for the visualization of flow and adsorption in microfluidic systems. The instrument consists of an inverted epifluorescence microscope, a solid-state laser, a high-speed CCD camera, a low-noise DC power supply, and some smaller equipment. Measurement of adsorption will be accomplished by total internal reflection fluorescence (TIRF). Microfluidic devices will be constructed using standard soft lithography methods, allowing for control of microchannel geometry and surface chemistry. Fluid flow will be induced either by electroosmosis or a syringe pump, depending on the application.Three research projects, representative of those to be supported by this equipment, are as follows: The first is an assessment of the contribution of lateral diffusion to the kinetics of DNA hybridization to surface-bound probes as means to improve the performance of biosensors. The second is the development of a microscale chromatography system that utilizes DNA-binding surfactants to isolate target DNA from complex mixtures. The third is the development and validation of numerical algorithms for improved computer-aided design of lab-on-a-chip systems.The impact of the instrumentation will be on many fronts. This is perhaps the first microfluidic visualization instrument that includes on-line TIRF, supplying surface adsorption information critical to the above research. In addition, the many groups on campus involved with microfluidics and MEMS would have this instrument as a shared resource. We will also perform a series of microfluidics experiments (available as a web-based resource) to serve as examples and problem sets for transport courses. We will also develop hands on projects for high school students participating in the Summer Academy for Minority Students at Carnegie Mellon University. This would serve as an introduction to an exciting field and familiarize students with state-of-the-art lab equipment.