Collaborative Research: Development and Field use of Cyanobacterial Iron-Dependent Bioreporters

鲍灵格林州立大学和克拉克森大学的研究人员将合作开发一系列荧光蓝藻铁依赖生物报告菌株，这些菌株能够量化水生环境中铁的生物利用度。该项目将使用相同的遗传技术和扩展的分析方法，用于开发淡水生物报告原型，其中含有蓝细菌聚藻球菌sp. PCC 7942。通过分析每个启动子产生的剂量-响应曲线，研究人员希望构建一系列具有扩大生物可利用铁检测动态范围的生物报告基因。虽然这项研究提供的信息将解决我们目前对淡水生物可获得铁的知识的空白，但研究人员希望它最终将适用于类似海洋传感器的开发。

ABSTRACTOCE-0327738OCE-0327730Researchers at Bowling Green State University and Clarkson University will collaborate to develop a series of luminescent cyanobacterial Fe-dependent bioreporter strains capable of quantifying the bioavailability of iron in aquatic environments. This project will use the same genetic techniques and expanded analytical approaches used to develop a prototype freshwater bioreporter with the cyanobacterium Synechococcus sp. PCC 7942. By analyzing dose-response curves generated for each promoter, the investigators hope to construct a series of bioreporters with an expanded dynamic range of detection of bioavailable iron. While the information provided from this study will address a gap in our current knowledge on the availability of iron to freshwater organisms, the investigators expect that it will eventually be applicable to the development of analogous marine sensors.