CAREER: Regulation of Monoclonal Antibody Production in Murine Hybridomas

该项目的目的是研究在培养的杂交瘤细胞中抗体产生的调节机制。具体的项目目标是：(1)确定对渗透胁迫的反应中抗体产生的增加是否受转录、翻译或翻译后过程的调节；(2)评估渗透胁迫反应中应激激活的和丝裂原激活的蛋白激酶级联反应的活性；(3)确定应激反应蛋白和伴侣蛋白在渗透胁迫反应中的作用；(4)确定细胞周期在渗透胁迫反应中的作用；以及(5)使用现有的抗体合成的数学模型来确定抗体合成中的限速步骤。

The objective of this project is to investigate the mechanism by which antibody production is regulated in cultured hybridoma cells. The specific project objectives are to: (1) determine whether the increase in antibody production in response to osmotic stress is regulated by transcription, translation or post-translational processes, (2) evaluate activities of the stress-activated and mitogen-activated protein kinase cascades in response to osmotic stress, (3) establish the role of stress response proteins and chaperones in the osmotic stress response, (4) determine the role of cell cycle in osmotic stress response, and (5) use existing mathematical models of antibody synthesis to determine rate limiting steps in antibody synthesis.