Microbial Genome Sequencing:Wheat Stem Rust Fungus Genome Sequencing Project

锈菌是专性植物寄生虫，其寄主范围广泛，包括蕨类植物、裸子植物和被子植物。小麦锈菌。小麦茎锈病的病原小麦曲曲菌是小麦最具破坏性的真菌病原体。20世纪50年代初的流行病摧毁了北美超过4亿蒲式耳的小麦。该项目的结果将使使用基因组学和其他方法来阐明锈菌的致病性机制和生物营养生活方式的本质。本研究的具体目的是：(1)获得一株禾本科单胞菌的基因组序列。小麦至少有8倍的覆盖率；(2)利用DNA指纹图谱建立禾草种子的物理图谱；(3)从代表真菌发育4个不同阶段的cDNA全长文库中提取40000个ESTs；(4)将EST、遗传和物理制图数据与组装序列相结合；(5)对基因组序列进行注释，并通过用户友好的网络工具和界面将这些数据公开；(6)培养生物信息学和真菌基因组学方向的本科生和博士后科研人员。

Rust fungi (Uredinales) are obligate plant parasites with a wide range of plant hosts that include ferns, gymnosperms and angiosperms. Puccinia graminis f.sp. tritici, the causal agent of stem rust of wheat, has been the most devastating fungal pathogen of wheat. Epidemics of the early 1950's destroyed more than 400 million bushels of wheat in North America. The results of this project will enable the use of genomic and other approaches to elucidate the mechanisms of pathogenicity and the nature of the biotrophic life-style of rust fungi. The specific objectives of this research are: (1) to produce an assembled genome sequence of one isolate of P. graminis f.sp. tritici with at least 8X coverage; (2) to develop a physical map of P. graminis via DNA fingerprinting fosmid clones; (3) to develop 40,000 ESTs from full length cDNA libraries representing four different stages of fungal development; (4) to integrate EST and, genetic and physical mapping data with the assembled sequence; (5) to annotate the genomic sequence and make these data publicly available through user friendly web-based tools and interfaces; and (6) to train undergraduate students and Postdoctoral Research Fellows in bioinformatics and fungal genomics.