Microbial Genome Sequencing: Genome Sequencing and Analysis of Bacillus Pumilis

本项目的目标是产生一个完整的、封闭的和完整的短芽孢杆菌菌株FO-036b的基因组序列。该菌株产生孢子和营养细胞，对各种灭菌剂（包括气相过氧化氢、紫外线辐射、伽马辐射和干燥）的抗性显著提高。这些形成孢子的细菌对医疗、制药、半导体制造、航天器组装和食品加工环境的适当维护提出了挑战。该项目将在贝勒医学院人类基因组测序中心进行。策略将是执行全基因组鸟枪测序克隆不同大小的插入。装配、整理和注释将使用BCM-HGSC现有的程序。最终组装将通过在基因组上形成连续平铺路径的克隆的指纹来验证。序列和装配信息将立即通过BCM-HGSC的网站公开。该项目将促进对抗紫外线辐射和气相过氧化氢的遗传基础的基础研究，以及了解生物体如何适应高氧化环境。该项目将引起研究团体的兴趣，包括那些对孢子和DNA修复感兴趣的团体，关注行星保护和生命适应高紫外线通量或氧化环境的NASA团体，需要维持清洁环境的工业团体以及关注高抗性病原体的生物防御团体。

The goal of this project is to produce a complete, closed and finished genome sequence of the sometimes-pathogenic Bacillus pumilus strain FO-036b. This strain produces spores and vegetative cells with dramatically elevated resistance to a variety of sterilizing agents, including vapor phase hydrogen peroxide, UV radiation, gamma radiation and desiccation. Such spore forming bacteria challenge proper maintenance of environments in medical, pharmaceutical, semiconductor manufacturing, spacecraft assembly, and food processing environments. The project will be performed at the Baylor College of Medicine Human Genome Sequencing Center. The strategy will be to perform whole genome shotgun sequencing of clones with different sized inserts. Assembly, finishing, and annotation will use the existing procedures at the BCM-HGSC. Final assembly will be verified by fingerprinting of clones that form a contiguous tiling path on the genome. Sequence and assembly information will be made public via a website at the BCM-HGSC without delay. This project will facilitate basic research on the genetic basis for resistance to UV radiation and vapor phase hydrogen peroxide as well as understanding how organisms adapt to highly oxidizing environments. The project will be of interest to research communities including those interested in sporulation and DNA repair, NASA communities concerned with planetary protection and adaptation of life to high UV flux or oxidizing environments, industrial communities that require maintenance of clean environments and the biodefense community concerns about highly resistant pathogenic agents.