Infrared Matrix Assisted Laser Desorption Ionization Mass Spectrometry

路易斯安那州立大学的Kermit Murray教授得到分析和表面化学项目的支持，以进一步开发和应用红外基质辅助激光解吸电离质谱（IR-MALDI）。 IR-MALDI不像使用紫外线（UV）辐射的MALDI那样被广泛接受，但提供了一些优点，包括产生具有较少内能的离子的能力、在不使用基质的情况下产生离子的能力以及在没有低质量干扰的情况下表征低分子量分析物的能力。红外激光解吸，双脉冲红外/紫外MALDI，和粒子消融的基础研究正在进行的是“直接从凝胶”红外激光解吸电离策略肽表征的发展。 PI使用一种新颖的基于网络的虚拟实验室之旅向大学生介绍质谱，并向公众介绍科学研究人员的日常工作。各种形式的质谱已被证明对生物分子的高通量高精度分析至关重要。 鉴于UV-MALDI-MS技术作为一种灵敏的分析技术（特别是在蛋白质组学和越来越多的生物医学分析中）的高度影响，IR-MALDI的前景是光明的。 该项目概述了生物分子质谱领域的仪器和技术的改进，以及新方法和应用的开发，这些方法和应用应扩大MALDI应用的作用，如生物医学研究，临床测量和合成聚合物。

Professor Kermit Murray of Louisiana State University is supported by the Analytical and Surface Chemistry Program to further the development and application of infrared matrix-assisted laser desorption ionization mass spectrometry (IR-MALDI). IR-MALDI is not as widely accepted a method as MALDI using ultraviolet (UV) radiation, but offers some advantages including the ability to produce ions with less internal energy, the ability to produce ions without the use of a matrix, and the ability to characterize low molecular weight analytes without low mass interferences. Fundamental studies of IR laser desorption, two-pulse IR/UV MALDI, and particle ablation are being conducted as is the development of "direct from gel" IR laser desorption ionization strategies for peptide characterization. The PI uses a novel web-based Virtual Lab Tour to introduce college students to mass spectrometry and to introduce the public to what scientific researchers do on a daily basis.Mass spectrometry in various forms has proven essential to the high-throughput highly accurate analysis of biological molecules. Given the high impact the UV-MALDI-MS technique has as a sensitive analytical technique (in particular in proteomics and increasingly in biomedical analysis), prospects for IR-MALDI are promising. This project outlines improvement of instrumentation and techniques in the area of mass spectrometry of biological molecules, as well as development of new approaches and applications that should expand the role of MALDI applications such as biomedical research, clinical measurements, and synthetic polymers.