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Infrared Laser Ablation Microsampling for Mass Spectrometry Imaging

用于质谱成像的红外激光烧蚀微量采样

基本信息

批准号：
1709526
负责人：
Kermit Murray
金额：
$ 34.84万
依托单位：
Louisiana State University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-08-01 至 2021-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1709526&HistoricalAwards=false
关键词：
Infrared Laser Ablation Microsampling Mass

项目摘要

This project is funded by the Chemical Measurement and Imaging program of the Chemistry Division. Professors Kermit Murray of Louisiana State University (LSU) and Professor Touradj Solouki of Baylor University are developing techniques for looking at (imaging) biological tissue in order to identify and quantify the biomolecules in the samples. Tissue imaging is often limited to the most abundant compounds and cannot identify important but less abundant molecules that may be key factors for biological function and disease diagnostics. This project uses lasers for direct sampling of tissue as well as precision microdissection for detailed analysis. The ability to localize, identify, and quantify biomolecules in tissue is important to fundamental research and to clinical diagnostics - especially when it is important to monitor the progress of treatment. The outreach program includes a novel, open education initiative creating Web-based resources for education using the visualization tools developed in this research. Educational opportunities are provided to high school, undergraduate, and graduate students. LSU has a strong history of quality mentorship for underrepresented students, especially African Americans. That quality mentorship is continued and advanced in this work. This project is aimed at the development of a comprehensive approach to mass spectrometry imaging that is quantitative and capable of biomolecule identification. Matrix-assisted laser desorption ionization mass spectrometry imaging is combined with infrared laser ablation sample transfer for liquid chromatography tandem mass spectrometry analysis. The objectives are construction of an infrared laser ablation tissue microprobe, combining MALDI imaging with laser ablation sample transfer, and investigation of the fundamental processes of tissue laser ablation for technique optimization. The educational goals support graduate student training at LSU and Baylor and is promoting collaborative interactions between these institutions. This work has societal benefits through the development of a broadly applicable approach to biological imaging at the molecular level with applications. Education and outreach components of this project involve the development of open resources for mass spectrometry education and research. These components include creating collaborative resources for mass spectrometry on Wikipedia and integration of Human Proteome Organization (HUPO) controlled vocabulary with terminology developed by the International Union of Pure and Applied Chemistry.

该项目由化学部化学测量和成像项目资助。路易斯安那州立大学 (LSU) 的 Kermit Murray 教授和贝勒大学的 Touradj Solouki 教授正在开发观察生物组织（成像）的技术，以便识别和量化样本中的生物分子。组织成像通常仅限于最丰富的化合物，无法识别重要但含量较低的分子，而这些分子可能是生物功能和疾病诊断的关键因素。该项目使用激光直接对组织进行采样，并使用精密显微切割进行详细分析。定位、识别和量化组织中生物分子的能力对于基础研究和临床诊断非常重要，尤其是当监测治疗进展很重要时。该推广计划包括一项新颖的开放式教育计划，使用本研究中开发的可视化工具创建基于网络的教育资源。向高中生、本科生和研究生提供教育机会。路易斯安那州立大学在为代表性不足的学生（尤其是非裔美国人）提供高质量指导方面有着悠久的历史。这项高质量的指导在这项工作中得到了延续和推进。该项目旨在开发一种定量且能够识别生物分子的质谱成像综合方法。基质辅助激光解吸电离质谱成像与红外激光烧蚀样品转移相结合，用于液相色谱串联质谱分析。目标是构建红外激光烧蚀组织微探针，将 MALDI 成像与激光烧蚀样品转移相结合，并研究组织激光烧蚀的基本过程以优化技术。教育目标支持路易斯安那州立大学和贝勒大学的研究生培训，并促进这些机构之间的协作互动。这项工作通过开发一种广泛适用的分子水平生物成像方法和应用来产生社会效益。该项目的教育和推广部分涉及为质谱教育和研究开发开放资源。这些组成部分包括在维基百科上创建质谱分析协作资源，以及将人类蛋白质组组织 (HUPO) 控制的词汇与国际纯粹与应用化学联盟开发的术语相集成。

项目成果

期刊论文数量（12）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Piezoelectric matrix-assisted ionization

压电矩阵辅助电离

DOI：
10.1177/1469066718816696
发表时间：
2019
期刊：
European Journal of Mass Spectrometry
影响因子：
1.3
作者：
Banstola, Bijay;Szot, Carson W;Deenamulla Kankanamalage, Achala P;Murray, Kermit K
通讯作者：
Murray, Kermit K

Broadband ion mobility deconvolution for rapid analysis of complex mixtures

用于快速分析复杂混合物的宽带离子淌度解卷积

DOI：
10.1039/c8an00193f
发表时间：
2018
期刊：
The Analyst
影响因子：
0
作者：
Pettit, Michael E.;Brantley, Matthew R.;Donnarumma, Fabrizio;Murray, Kermit K.;Solouki, Touradj
通讯作者：
Solouki, Touradj

A nanoparticle co‐matrix for multiple charging in matrix‐assisted laser desorption ionization imaging of tissue

用于在基质中多次充电的纳米颗粒共基质辅助激光解吸电离组织成像