SENSORS: Rapid, Culture-Based Sensor with Direct Electronic Imaging to Track Environmental Microorganisms

0428600 Papautsky这项研究的目标是开发一种具有微流控样品制备和直接电子成像的基于培养的传感器，用于快速跟踪环境微生物，并展示这一新的传感器技术作为现有微生物监测工具的有效替代。拟议研究的具体目标有三个方面：(1)确定影响微生物在微型培养传感器上选择性黏附和生长的因素；(2)将培养传感器技术与微流控平台和直接电子成像相结合，以快速追踪微生物；(3)基准培养传感器与基于分子生物学的工具进行正面比较，以鉴定和定量环境样品中含有革兰氏阳性、霉菌酸的放线菌(如分枝杆菌和诺卡氏菌)。

0428600PapautskyThe goal of this research is to develop a culture-based sensor with microfluidic sample preparation and direct electronic imaging for rapid tracking of environmental microorganisms and to demonstrate this new sensor technology as an effective replacement of existing microbial monitoring tools. The specific aims of the proposed research are three-fold: (1) identify factors that affect selective adhesion and growth of microorganisms on miniaturized, culture-based sensors; (2) integrate culture-based sensor technology with a microfluidic platform and direct electronic imaging to rapidly track microorganisms; and (3) benchmark culture-based sensor in head-to-head comparisons with molecular biology-based tools to identify and quantify Gram positive, mycolic acid containing Actinomycetes (e.g., mycobacteria and nocardioforms) in environmental samples.