ACT/SGER: Technology for Field Portable Biosensors

该项目将开发基于自由基聚合的技术，用于扩增生物分子识别事件，特别是寡核苷酸杂交，这样检测这些事件将更容易，高度平行，在降低事件阈值浓度下可行，并且可以用廉价的检测器检测到。自由基聚合为放大生物分子识别事件提供了一个独特的机会，因为每个起始事件都会导致大量乙烯基的聚合。当起始在空间或时间上与寡核苷酸识别事件耦合时，高吸收部分的聚合为扩增和随后的吸收检测提供了手段，使设计简单，便携式检测器成为可能。该奖项由美国国家科学基金会和情报界共同支持。数学和物理科学理事会的反恐方法项目支持基础研究和劳动力发展方面的新概念，这些新概念有可能为国家安全做出贡献。

This project will develop radical polymerization-based techniques for amplification of biomolecular recognition events, specifically olignucleotide hybridization, such that detection of these events will be more facile, highly parallel, feasible at reduced event threshold concentrations, and detectable with an inexpensive detector. Radical polymerizations afford a unique opportunity to amplify biomolecular recognition events since each initiation event leads to the polymerization of a large number of vinyl groups. When initiation is, in space or time, coupled to the oligonucleotide recognition event, polymerization of highly absorptive moieties provide means for amplification and subsequent detection by absorption, enabling the design of a simple, portable detector. This award is supported jointly by the NSF and the Intelligence Community. The Approaches to Combat Terrorism Program in the Directorate for Mathematics and Physical Sciences supports new concepts in basic research and workforce development with the potential to contribute to national security.