Electrostatically Bound Protein-Lipid Bilayer Domains

在这个由化学学部实验物理化学项目资助的项目中，威斯康星大学麦迪逊分校的James C. Weisshaar教授和他的研究生将使用物理化学的方法来试图理解细胞的细胞质膜中负责胞外分泌的基本机制。主要工具将是在合成平面脂质双分子层上移动蛋白分子的单分子荧光显微镜。所开发的方法应该适用于其他有趣的生物物理系统。除了拟议的研究对其他生物物理系统的潜在更广泛的影响外，从事该项目的年轻研究人员将接触到独特的跨学科经验。此外，Weisshaar教授将把他的研究纳入他所教授的本科课程，他将访问当地的社区组织，以促进对现代研究型大学的更好理解。

In this project, funded by the Experimental Physical Chemistry Program of the Chemistry Division, Prof. James C. Weisshaar of the University of Wisconsin - Madison and his graduate research students will use the methods of physical chemistry to attempt to understand the fundamental mechanisms in a cell's plasma membrane responsible for exocytosis. The primary tool will be single-molecule fluorescence microscopy of mobile protein molecules on a synthetic planar lipid bilayer. The methods developed should be applicable to other interesting biophysical systems.Besides the potential broader impact of the proposed research on other biophysical systems, the young researchers working on this project will be exposed to a unique interdisciplinary experience. In addition, Prof. Weisshaar will incorporate aspects of his research into the undergraduate courses that he teaches, and he will visit local community organizations to promote better understanding of the modern research university.