SoyMap: an Integrated Soybean Genome Map

该奖项的长期目标是使用大豆基因组工具破译多轮多倍化之后的二倍化的遗传/基因组效应，这是一种鲜为人知的现象，并为公众开发基于BAC的序列就绪图谱。为了开始实现这些目标，该团队将创建一个与现有遗传图谱整合的物理图谱。物理图谱是用于克隆重要农业基因和对基因组片段或整个基因组进行测序的基本构件。除了开发一个完整的遗传-物理图谱外，还将进行DNA测序，以评估几个地点的大豆基因组的微结构。大豆基因组是高度复制的；也就是说，任何特定区域可能会多次出现，这使得基因组图谱和测序变得复杂。该团队将针对复制区域并对其进行排序，以确定复制区域之间的保守程度，并确定如何克服基因组内复制带来的困难。在该项目中，主要是本科院校的教职员工和学生每年夏天都有机会参加每个相关院校的项目。获取项目成果所有DNA序列将保存在GenBank中，并在质量检查后立即保存在跟踪文件存储库中的跟踪文件。综合物理和遗传图谱数据将通过豆科植物信息系统(LIS：http://www.comparative-legumes.org/).)提供

The long-term goals of this award are to use genomic tools in soybean to decipher the genetic/genomic effects of multiple rounds of polyploidization followed by diploidization, a poorly understood phenomenon, and develop a BAC-based, sequence ready map for the public. To begin to address the goals, the team will create a physical map integrated with the available genetic map. A physical map is the basic building block used to clone important agricultural genes and to sequence segments of genomes or entire genomes. In addition to development of an integrated genetic-physical map, DNA sequencing will be done to assess the microstructure of the soybean genome at several locations. The soybean genome is highly duplicated; that is, any particular region may be present multiple times, which complicates genome mapping and sequencing. The team will target and sequence the duplicated regions to ascertain the level of conservation between duplicated regions and to determine how to overcome the difficulty posed by duplications within the genome. Integrated into the project are opportunities for faculty and students from Primarily Undergraduate Institutions to participate each summer in the project at each of the involved institutions. Access to project outcomesAll DNA sequences will be deposited in GenBank and trace files in the trace file repository as soon as the qualities are checked. Integrated physical and genetic map data will be made available through the Legume Information System (LIS: http://www.comparative-legumes.org/).