Leveraging Untapped Genetic Diversity in Soybean

主要研究者：斯科特杰克逊（普渡大学）CoPIs：Randy Shoemaker（爱荷华州州立大学/USDA-ARS），杰里米·施穆茨（哈德逊阿尔法研究所，亨茨维尔，亚拉巴马），杰夫道尔（康奈尔大学），佩里克里根（马里兰州大学-学院公园/USDA-ARS）高级人员：简·格里姆伍德（哈德森阿尔法研究所，亨茨维尔，亚拉巴马），Jianxin Ma和Jessica Schlutter（普渡大学）大豆（Glycine max）是美国燃料和饲料经济的重要组成部分。然而，大豆的遗传改良受到缺乏遗传多样性的困扰，使大豆受到害虫、病原体和非生物胁迫的威胁。该项目将开发一套工具，使研究人员能够利用最近发布的大豆基因组序列草案，利用大豆属的遗传多样性，并开始了解该属物种/基因组多样化的性质。在这一过程中重要的是了解在这组物种中发现的多倍体化和二倍体化的循环。具体目标是：1）利用一组具有遗传信息的大豆属物种的基因组克隆将多样性图谱覆盖到大豆基因组序列上; 2）在染色体/序列水平上探索大豆属的基因组进化，以了解多倍性/二倍体化循环;第三章为了了解连锁不平衡和遗传多样性的水平，可用于遗传学和育种;以及4）将数据与其他豆类整合，这对于将其有用性扩展到其他所谓的"孤儿"豆类如菜豆是必要的。该项目的更广泛影响将是开发一个基础设施，使更广泛的豆类社区能够普遍利用这一经济重要属的多样性和了解基因组进化。该项目将继续并扩大一个成功的外展计划，目标是在西南部（主要是新墨西哥州和亚利桑那州）的美洲原住民和西班牙裔本科院校，以提供夏季研究培训机会，并进一步发展与这些教师和学生的互动。 所有项目数据和工具都可以通过www.example.com的数据库访问。 序列数据将通过基因库储存和检索，BAC克隆资源将由亚利桑那基因组研究所在成本回收的基础上维护和分发。

PI: Scott Jackson (Purdue University)CoPIs: Randy Shoemaker (Iowa State University/USDA-ARS), Jeremy Schmutz (HudsonAlpha Institute, Huntsville, Alabama), Jeff Doyle (Cornell University), Perry Cregan (University of Maryland - College Park/USDA-ARS)Senior Personnel: Jane Grimwood (HudsonAlpha Institute, Huntsville, Alabama), Jianxin Ma and Jessica Schlueter (Purdue University)Soybean (Glycine max) is an important part of the US fuel and feed economy. However, genetic improvement of soybean is beset by a lack of genetic diversity leaving soybean open to threats of pests, pathogens and abiotic stresses. This project will develop a set of tools that will allow investigators to leverage the recently released draft soybean genome sequence to exploit genetic diversity in the genus Glycine and to begin to understand the nature of species/genome diversification in the genus. Important in this process is an understanding of the cycle of polyploidization followed by diploidization found in this set of species. The specific objectives are to: 1) overlay diversity maps onto the soybean genome sequence using genomic clones from a set of phylogenetically informative Glycine species; 2) explore genome evolution in the genus Glycine at the chromosomal/sequence level to understand the polyploidy/diploidization cycle; 3) sequence targeted orthologous regions across the Glycine species in order to understand levels of linkage disequilibrium and genetic diversity that can be exploited for genetics and breeding; and 4) integrate data with other legumes which is necessary to extend its usefulness to other so-called "orphan" legumes such as common bean. The broader impacts of this project will be the development of an infrastructure that will be generally accessible by the broader legume community to exploit diversity and understand genome evolution in this economically important genus. The project will continue and expand a successful outreach program targeted toward Native American and Hispanic undergraduate institutions in the Southwest (primarily New Mexico and Arizona) to provide summer research training opportunities and further develop interactions with these faculty and students. All project data and tools will be accessible though SoyBase at http://soybase.org/. Sequence data will be deposited and accessible through GenBank and BAC clone resources will be maintained and distributed on a cost-recovery basis by the Arizona Genome Institute.