Leveraging Untapped Genetic Diversity in Soybean

利用大豆未开发的遗传多样性

• 批准号: 0822258
• 负责人: Scott Jackson
• 金额: --
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0822258&HistoricalAwards=false
• 关键词: Leveraging; Untapped; Genetic; Diversity; Soybean

PI: Scott Jackson (Purdue University)CoPIs: Randy Shoemaker (Iowa State University/USDA-ARS), Jeremy Schmutz (HudsonAlpha Institute, Huntsville, Alabama), Jeff Doyle (Cornell University), Perry Cregan (University of Maryland - College Park/USDA-ARS)Senior Personnel: Jane Grimwood (HudsonAlpha Institute, Huntsville, Alabama), Jianxin Ma and Jessica Schlueter (Purdue University)Soybean (Glycine max) is an important part of the US fuel and feed economy. However, genetic improvement of soybean is beset by a lack of genetic diversity leaving soybean open to threats of pests, pathogens and abiotic stresses. This project will develop a set of tools that will allow investigators to leverage the recently released draft soybean genome sequence to exploit genetic diversity in the genus Glycine and to begin to understand the nature of species/genome diversification in the genus. Important in this process is an understanding of the cycle of polyploidization followed by diploidization found in this set of species. The specific objectives are to: 1) overlay diversity maps onto the soybean genome sequence using genomic clones from a set of phylogenetically informative Glycine species; 2) explore genome evolution in the genus Glycine at the chromosomal/sequence level to understand the polyploidy/diploidization cycle; 3) sequence targeted orthologous regions across the Glycine species in order to understand levels of linkage disequilibrium and genetic diversity that can be exploited for genetics and breeding; and 4) integrate data with other legumes which is necessary to extend its usefulness to other so-called "orphan" legumes such as common bean. The broader impacts of this project will be the development of an infrastructure that will be generally accessible by the broader legume community to exploit diversity and understand genome evolution in this economically important genus. The project will continue and expand a successful outreach program targeted toward Native American and Hispanic undergraduate institutions in the Southwest (primarily New Mexico and Arizona) to provide summer research training opportunities and further develop interactions with these faculty and students. All project data and tools will be accessible though SoyBase at http://soybase.org/. Sequence data will be deposited and accessible through GenBank and BAC clone resources will be maintained and distributed on a cost-recovery basis by the Arizona Genome Institute.

PI：Scott Jackson（普渡大学）CoPI：Randy Shoemaker（爱荷华州立大学/USDA-ARS）、Jeremy Schmutz（HudsonAlpha 研究所，亨茨维尔，阿拉巴马州）、Jeff Doyle（康奈尔大学）、Perry Cregan（马里兰大学帕克分校/USDA-ARS）高级人员：Jane Grimwood（HudsonAlpha 研究所，亨茨维尔， 阿拉巴马州）、Jianxin Ma 和 Jessica Schlueter（普渡大学）大豆 (Glycine max) 是美国燃料和饲料经济的重要组成部分。然而，大豆的遗传改良受到遗传多样性缺乏的困扰，使大豆容易受到害虫、病原体和非生物胁迫的威胁。该项目将开发一套工具，使研究人员能够利用最近发布的大豆基因组序列草案来开发大豆属的遗传多样性，并开始了解该属物种/基因组多样化的本质。在此过程中重要的是了解这组物种中发现的多倍化和二倍化的循环。具体目标是：1）使用来自一组系统发育信息丰富的大豆物种的基因组克隆，将多样性图谱叠加到大豆基因组序列上； 2）在染色体/序列水平上探索大豆属的基因组进化，以了解多倍体/二倍体化循环； 3) 对大豆物种的定向直系同源区域进行测序，以了解可用于遗传和育种的连锁不平衡水平和遗传多样性； 4）将数据与其他豆类整合，这对于将其有用性扩展到其他所谓的“孤儿”豆类（例如普通豆类）是必要的。该项目的更广泛影响将是开发一个基础设施，更广泛的豆类社区可以普遍使用该基础设施，以利用多样性并了解这个经济上重要的属的基因组进化。该项目将继续并扩大针对西南部（主要是新墨西哥州和亚利桑那州）的美洲原住民和西班牙裔本科院校的成功外展计划，以提供夏季研究培训机会并进一步发展与这些教师和学生的互动。 所有项目数据和工具都可以通过 SoyBase（http://soybase.org/）访问。 序列数据将通过 GenBank 进行存储和访问，BAC 克隆资源将由亚利桑那州基因组研究所在成本回收的基础上进行维护和分发。