Multifunctional colloidal nanoparticles for dual imaging with fluorescence, magnitic and radioactive detection

由无机核和聚合物壳组成的胶体纳米粒子将被合成。核和聚合物外壳要么是荧光的，要么是磁性的，要么是放射性的，这样它们就可以分别用荧光、磁共振或放射性成像。通过将不同的核与不同的聚合物壳相结合，将获得九种不同类型的粒子进行双重成像，例如荧光核与放射性聚合物壳。通过这种方式，一个包含9种纳米粒子的工具包将被创造出来，每一种纳米粒子都可以用两种不同的模式成像。由于聚合物外壳的地形，所有九种不同类型的颗粒将具有相同的表面化学性质，因此具有与配体连续偶联的相同界面。双重标记将允许检查被活细胞结合的纳米颗粒的命运，例如，发现核心周围的聚合物外壳是否被消化或释放。双重标记的示范实验将涉及器官（在整个动物水平上）、器官来源的组织（在组织水平上）以及组织来源的细胞（在单细胞水平上）内颗粒分布的全频带宽度。

Colloidal nanoparticles composed out of an inorganic core and a polymer shell will be synthesized. Both, the core and the polymer shell will be either fluorescence, magnetic, or radioactive, so that they can be imaged with fluorescence, magnetic resonance, or radioactivity, respectively. By combining different cores with different polymer shells nine different types of particles for dual imaging will be obtained, as for example fluorescent cores with radioactive polymer shells. In this way a toolkit of nine types of nanoparticles will be created out of which each can be imaged with two different modes. Due to the topography of the polymer shell all nine different types of particles will posses the same surface chemistry and thus have the same interface for consecutive conjugation with ligands. Double labelling will allow for examining the fate of nanoparticles which are incorporated by living cells, for example to find out if the polymer shell around the core is digested or released. Demonstration experiments with double labelling will involve the full band width of particle distribution within organs (on a whole animal level), within tissue derived from the organs (on a tissue level) up to distribution within cells derived from the tissue (on a single cell level).