Microbial Genome Sequencing: Genome Sequencing and Analysis of Azotobacter Vinelandii

该项目有两个目标：制作完成的、注释良好的自由生活固氮菌棕色固氮细菌的基因组序列，以及在参与机构和场外机构扩大现有的基因组学技术本科培训平台。棕色假单胞菌(A.vinelandii)是假单胞菌科的伽马蛋白细菌。这种微生物提供了一个很好的系统来研究好氧固氮、包囊形成和异种生物降解的过程。在假单胞菌科中，只有三个主要属中的一个具有完整的序列代表(假单胞菌)。因此，提供完整的棕色拟青霉基因组将成为比较基因组学的基本黄金标准系统发育锚点之一。对固氮菌生物学这些方面和其他方面的研究将通过提供一个注释良好的基因组序列而得到显着加强。基因组的注释和分析将与本科生和更广泛的固氮菌研究社区密切合作。本科生研究人员将融入项目的所有方面，包括课堂内和通过独立的研究项目，包括整理、注释、生物信息学和分析阶段。这种参与不仅将为基因组学和生物信息学领域的新科学家提供关键的培训，而且将是实现关键项目目标的关键组成部分。根据每个目标，这个项目汇集了来自五个独立小组的广泛专业知识，这些小组在固氮菌生物学、基因组学、生物信息学和本科教育方面具有丰富的经验。

This project has two goals: The production of a finished, well-annotated genome sequence of the free-living nitrogen fixing bacterium Azotobacter vinelandii and the expansion of existing undergraduate training platforms in genomics technology at both participating and off-site institutions. A. vinelandii is a gamma-proteobacterium belonging to the family Pseudomonadaceae. This organism provides an excellent system in which to examine the processes of aerobic nitrogen fixation, cyst formation and xenobiotic degradation. Within the Pseudomonadaceae family only one of the three main genera has fully sequenced representatives (Pseudomonas). Provision of the complete A. vinelandii genome will therefore serve as one of the essential gold-standard phylogenetic anchor points for comparative genomics. Studies on these and other aspects of Azotobacter biology will be significantly enhanced by the availability of a well-annotated genome sequence. Annotation and analysis of the genome will occur in close collaboration with undergraduate students and the broader Azotobacter research community. Undergraduate researchers will be integrated into all aspects of the project, both within the classroom and through independent research projects, including the finishing, annotation, bioinformatics and analytical phases. Such participation will not only provide critical training for new scientists in the field of genomics and bioinformatics, it will be a key component by which critical project goals will be met. In line with each of these goals this project brings together extensive expertise from five independent groups with significant experience in Azotobacter biology, genomics, bioinformatics, and undergraduate education.