SST: Optimizing Microfluidic Transport and Magnetic Sensing for Detection of Detection of Pseudomonas Syringae

摘要：Abraham D. Stroock，James Engstrom和Kelvin Lee机构：康奈尔大学提案编号：0529042标题：SST：优化微流控质量转移和磁传感用于检测假单胞菌。该项目的首要目标是开发检测细菌性植物病原体-假单胞菌的技术。待开发的技术将依赖于检测该生物体发病过程中表达的效应子-辅助蛋白的能力。效应子-辅助蛋白的检测将依赖于嵌入微流体装置中的巨磁阻（GMR）传感器的使用。预计通过该项目开发的技术进步和传感器将广泛适用于许多生物系统的传感。该项目有以下具体目标：1）基本原则和微流体工具的开发，用于优化固体表面上的传感元件的质量传递。2）与巨磁阻传感兼容的磁性标签蛋白质传感器的蛋白质和表面化学的开发。3）使用磁性标签GMR传感器进行蛋白质检测的最佳微流体质量传递策略的实施。4）在康奈尔大学围绕微化学技术的物理，化学和实验原理构建跨学科研究社区和课程。通过REU活动，增加代表性不足的群体成员参与研究。化学和生物化学传感器的发展是一项巨大的技术挑战，因为传感元件必须不可避免地反映出必须被感测的分子物种中存在的一些无尽的多样性。尽管如此，存在必须针对所有感测策略优化的感测过程的一般物理和化学方面：1）感兴趣的物质（分析物）到检测点的质量转移，2）分析物的存在的灵敏检测，以及3）分析物的化学特异性识别。PI建议解决与基于表面的生物传感器的重要背景下的传感的这三个方面的优化相关的基本挑战。特别是，他们计划开发运输分析和微流体工具，以提高固体检测器表面的质量转移速率，开发蛋白质和表面化学，允许附着磁珠标记的蛋白质和抗体，从而实现高灵敏度检测，并开发新的惰性表面和基于剪切的机制，以限制非特异性信号，从而提高选择性。PI一直积极参与将缩小规模纳入化学工程课程。这些活动包括在化学工程课程中开发几门新的核心和选修课程，以强调工程分析在小规模设计中的重要作用。此外，该团队还参与了外联活动，将康奈尔大学社区聚集在一起，讨论与微型系统有关的问题和挑战。除了这种当地的影响，该团队还参加了包括“这是一个纳米世界”展览的活动，该展览已被70多万非科学家看到，他们一直是伦理研讨会的主要参与者，以考虑纳米技术的社会影响。

ABSTRACTPI: Abraham D. Stroock, James Engstrom and Kelvin LeeInstitution: Cornell UniversityProposal Number: 0529042Title: SST: Optimized Microfluidic Mass Transfer and Magnetic Sensing for Detection of Pseudomonas syringaeIntellectual Merit. The overarching objective of this project is to develop technology for the detection of Pseudomonas syringae, a bacterial plant pathogen. The technology that is to be developed will rely on the ability to detect an effector-helper protein expressed during pathogenesis of this organism. The detection of the effector-helper protein will rely on the use of giant magnetoresistive (GMR) sensors embedded in microfluidic devices. It is expected that the technical advances and sensors developed through this project will have broad applicability to the sensing of many biological systems. The project has the following specific aims:1) Development of fundamental principles and microfluidic tools for the optimization of mass transfer to sensing elements on solid surfaces.2) Development of protein and surface chemistry for a Magnetic Tag Protein Sensor that is compatible with giant magnetoresistant sensing.3) Implementation of optimal microfluidic mass transfer strategies for protein detection with Magnetic Tag GMR sensors.4) Construction of an interdisciplinary research community and curriculum at Cornell University based around the physical, chemical, and experimental principles of microchemical technology. Increased participation in research by members of underrepresented groups through REU activities. The development of chemical and biochemical sensors is a vast technological challenge, as the sensing elements must inevitably reflect some of the endless diversity that exists in the molecular species that must be sensed. Nonetheless, there are generic physical and chemical aspects of the sensing process that must be optimized for all sensing strategies: 1) Mass transfer for the species of interest (analyte) to the point of detection, 2) sensitive detection of the presence of the analyte, and 3) chemically specific recognition of the analyte. The PIs propose to address fundamental challenges associated with the optimization of these three aspects of sensing in the important context of surface based bio-sensors. In particular, they plan to develop transport analysis and microfluidic tools to increase the rates of mass transfer to solid detector surface, to develop protein and surface chemistries that allow for attachment of magnetic bead-labeled proteins and antibodies that allow for detection with high sensitivity, and to develop new inert surfaces and shear-based mechanisms to limit non-specific signal and thus improve selectivity.Broader Impact. ThePIs have been actively engaged in the incorporation of scale-down into the Chemical Engineering curriculum. These activities have included the development of several new core and elective courses in the chemical engineering curriculum to emphasize the important role of engineering analysis in small-scale design. Further, the team has been engaged in outreach activities that bring together the Cornell community to discuss issues and challenges related to microscale systems. Beyond this local impact, the team has participated in activities that include the "It's a NanoWorld" exhibit that has been seen by more than 700,000 nonscientists and they have been key players in an ethics workshop to consider the Societal Implications of Nanotechnology.