Nanotox: Gene Expression Profiling of Single-Walled Carbon Nanotubes: A Unique Safety Assessment Approach

人们越来越关注工程纳米粒子对环境和人类健康的潜在不利影响。工程纳米材料将在体外暴露于人类细胞中，其毒性将使用高通量基因表达微阵列的创新技术进行评估。有四个主要目标：a)编制暴露于单壁碳纳米管（SWNT）的人原代肺细胞的可重复基因表达谱；b)应用不同层次的计算分析来确定是否存在毒性；c)跨生物组织与先前来自人类皮肤细胞的数据（获得NSF SGER奖）进行比较；d)通过将传统的风险评估方法与新的毒物基因组学计算方法相结合，为预测毒性的“系统生物学”方法奠定基础。

0536679 Cunningham There is increasing concern about potential adverse effects of engineered nanoparticles (NP) on the environment and on human health. Engineered nanomaterials will be exposed to human cells in vitro and their toxicity will be assessed using the innovative technology of high throughput gene expression microarrays. There are four main objectives: a) to compile reproducible gene expression profiles of primary human lung cells exposed to single-walled carbon nanotubes (SWNT), b) to apply different tiers of computational analysis to determine if toxicity is present, c) to compare across biological tissues with previous data from human skin cells (obtained with NSF SGER award) and d) to lay the foundation for a "systems biology" approach for predictive toxicity by merging traditional risk assessment methods with new toxicogenomics computational methods.