SGER: Single Molecule-Radiation Interaction in Whispering-Gallery Mode Evanescent Field

国家科学基础论文编号：CTS-0541585论文编号：CTS-0541585主要研究员：郭志雄所属：罗格斯大学论文题目：SGER：回音廊模式消逝领域中的单分子-辐射相互作用该提案被作为一份主动提交给化学和运输系统司的小资助金探索性研究(SGER)类别，并随后被分配给热系统计划的热传输和热处理分单元。本项目的目的是了解单个分子与逝去辐射场的相互作用如何影响回音廊模式(WGM)的微共振，以便基于WGM的光学传感器可以被探索为一种新的分子探测工具。该项目的目标将是使用拟议的WGM微共振传感器模拟单一蛋白质和蛋白质-蛋白质相互作用的检测。纳米制造的最新进展使得在具有微米/纳米级别的物理尺寸的精细且均匀的微腔中考虑光学共振成为可能。这类设备实现了广泛的超灵敏和紧凑集成的传感器技术。这一探索性项目将检验和展示使用WGM微传感器对单个生物分子和分子相互作用进行无标记检测的可行性、能力和灵敏度。如果成功，这项探索性工作将带来进步，可能导致开发出灵敏、选择性强、稳定、响应时间快的实用设备。除了蛋白质，可以固定在WGM传感器上的其他配体的例子包括用于检测特定抗原存在的抗体、用于通过氢化捕获的DNA，或用于检测特定底物/配体的酶/受体。在食品安全分析和低水平化学品和毒素检测方面的潜在应用将有助于国家打击生物恐怖主义的总体战略。这项研究将对将参与该项目的研究生和本科生的教育产生影响。学生参与研究为他们提供了将他们的智力视野扩展到跨学科研究的机会，并促进了对工程专业学生的培训，为进入高科技劳动力做准备。研究成果将在专业期刊、国内和国际会议上广泛传播，造福于社区和整个社会。

NATIONAL SCIENCE FOUNDATIONABSTRACTProposal Number: CTS-0541585Principal Investigator: Guo, ZhixiongAffiliation: Rutgers UniversityProposal Title: SGER: Single Molecule-Radiation Interaction in Whispering Gallery Mode Evanescent Field The proposal was received as an unsolicited submission to the Chemical and Transport Systems Division in the Small Grants for Exploratory Research (SGER) category and was subsequently assigned to the Thermal Transport and Thermal Processing Sub-element of the Thermal Systems Program. The objective of this project is to understand how the interactions of single molecules with the evanescent radiation field affect the whispering-gallery mode (WGM) microresonances such that WGM-based optical sensors can be explored as a new tool for molecular probing. The project objectives will be to simulate the detection of single proteins and protein-protein interactions using the proposed WGM microresonant sensor. Recent advances in nanofabrication have made it feasible to consider optical resonances in fine and uniform microcavities having physical dimensions at the micro/nanometer levels. Such devices enable a wide array of ultra-sensitive and compactly integrated sensor technologies. This exploratory project will examine and demonstrate the feasibility, capability and sensitivity of label-free detection of single biomolecules and molecular interactions using WGM microsensors. If successful, this exploratory work would lead to advances that may lead to the development of practical devices that are sensitive, selective, and stable with rapid response time. In addition to proteins, other examples of ligands that can be immobilized on the WGM sensor include antibodies to sense the presence of specific antigens, DNA for capture through hydridization, or enzymes/receptors for the detection of specific substrates/ligands. The potential applications to food safety analysis and detection of low levels of chemicals and toxins would contribute to the nation's general strategy in combating bio-terrorism. The research will impact the education of graduate students and undergraduate students who will participate in the project. The participation of students in research offers them the opportunities to expand their intellectual horizon to interdisciplinary research and promotes the training of engineering students to prepare for the high-technology workforce. The research results will be widely disseminated in professional journals, and national and international conferences and benefit the community and society at large.