SGER: Feasibility Study of DNA-Directed Polymer Synthesis

SGER: dna定向聚合物合成的可行性研究：narian C., Seeman，首席研究员，纽约大学；william A. Goddard， III，联合研究员，加州理工学院；arajan Vaidehi，联合研究员，加州理工学院；james W. Canary，联合研究员，纽约大学；该项目的目标是利用我们已经开发的DNA纳米技术来构建一个类似核糖体的纳米机器，该机器可用于将有机单体集合转化为具有精确定义的组成、序列和性质的聚合物（~30米）。因此，我们希望能够制造用于各种用途的新型聚合物，其特异性和控制水平与细胞用于制造导致特定功能的定义序列的蛋白质相同。除了组装具有新特征的聚合物外，我们的方法还将允许构建反映生化逻辑程序历史的分子，最终导致一个反射性化学合成系统，该系统对反映系统环境的信号做出反应。对于这里提出的探索性研究，我们将演示以下原理证明：[1]Seeman实验室将演示具有固定消息的原始翻译装置；该设备将简化系统中悬垂聚合物组件的使用和耦合的原型设计和故障排除。godard - vaidehi实验室将使用第一性原理计算方法来优化反应物、产物和偶联反应的过渡状态，以使设计具有正确间距的DNA模板以适应聚合反应的过渡状态。金丝雀实验室将通过实验证明，将DNA中的聚合物从DNA中移除到模板聚合物是可能的。我们已经开发了一种序列依赖旋转装置，它可以将信息从一种化学状态翻译成另一种化学状态，将DNA信号翻译成独立的DNA序列。现在，我们希望扩展这种转化能力，以构建具有实用价值的分子，例如非线性光学器件。我们期望，制造具有不同化学性质的精确定义片段的聚合物的能力，将构成在聚合物水平上控制物质结构的革命性步骤，包括精确控制聚合物的性质。更广泛的影响：所有研究人员都与少数民族研究生密切合作；这种互动在加州理工学院尤其根深蒂固。此外，他们都在实验室里训练高中生或本科生。这些活动通过与教师和研究科学家的密切互动来提高和促进学生的职业生涯。纽约大学的研究人员已经建立了一个很受欢迎的纳米技术讨论小组，现在已经是第四年了，来自纽约地区的学生和科学家都参加了这个小组。西曼与以科学为导向的艺术和娱乐团体密切合作，目前是新成立的国际纳米科学、计算和工程学会的主席。它寻求为纳米技术科学家和学生提供一个科学家园，使他们能够获得不易从建立在传统学科基础上的社会获得的认可和支持。

SGER: A Feasibility study of DNA-Directed Polymer Synthesis:Nadrian C., Seeman, Principal Investigator, New York UniversityWilliam A. Goddard, III, Co-Investigator, CaltechNagarajan Vaidehi, Co-Investigator, CaltechJames W. Canary, Co-Investigator, New York UniversityGoals and Intellectual Merit: The goal of this project is to use DNA nanotechnology we have developed to construct a ribosome-like nanoscale machine that can be used to convert a collection of organic monomers to polymers (~30mers) with precisely defined composition, sequence, and properties. Thus, we wish to be able to make novel polymers for a variety of purposes with the same level of specificity and control that the cell uses to make proteins of defined sequences that lead to particular functions. In addition to assembling polymers with novel features, our approach will allow the construction of molecules that reflect the history of a biochemical logic program, leading ultimately to a reflexive chemical synthetic system that builds constructs responsive to signals reflecting the environment of system. For the exploratory research proposed here, we will demonstrate the following proofs of principle: [1] The Seeman laboratory will demonstrate a primitive translation device with a fixed message; this device will simplify prototyping and trouble-shooting the use and coupling of pendant polymer components within the system. [2] The Goddard-Vaidehi laboratory will use first principles computational methods to optimize the reactants, the products, and the transition state of coupling reactions to enable the design of DNA templates with correct spacings to fit the transition state of the polymerization reaction. [3] The Canary laboratory will demonstrate experimentally that it is possible to remove polymers from the DNA in the DNA to template polymers.We have already developed a sequence dependent rotary device that prototypes the translation of information from one chemical state to another, translating DNA signals into independent DNA sequences. Now, we wish to extend this translational capability to build molecules whose implementation is of practical value, for example, non-linear optical devices. We expect that the capability to make polymers with precisely defined segments of varied chemical properties to constitute a revolutionary step towards controlling the structure of matter at the polymer level, including precise control of polymer properties.Broader Impact: All of the investigators work closely with minority graduate students; such interactions are particularly well-established at Caltech. In addition, all train high school or undergraduate students in their laboratories. These activities enhance and further student careers through close interaction with faculty and research scientists. The NYU investigators have established a well-attended nanotechnology discussion group now in its fourth year that is well-attended by students and scientists from the NYC area. Seeman works closely with scientifically-oriented arts and entertainment groups, and is currently serving as the president of the newly-founded International Society for Nanoscale Science, Computation, and Engineering. It seeks to provide a scientific home for nanotechnology scientists and students, so that they may obtain the recognition and support not readily available from societies founded on traditional disciplines.