Biophotonics: Quantum dots for single molecule imaging in live cells

0620813模拟这项提案中的工作将优化细胞中跟随单一蛋白质的荧光团。这可以显著提高研究蛋白质在许多不同的细胞作用中的作用的能力。有一些有机荧光团可以与蛋白质单价连接。不幸的是，它们不够光稳定，不能用于许多生物学研究。量子点具有明显更高的亮度和光稳定性。然而，它们聚集在一起，在它们的联系中是多价的。该项目将优化量子点的成像技术和设计，以减少聚集，并确保它们与蛋白质的连接是单价的。

0620813SimonThe work in this proposal will optimize fluorophores for following single proteins in cells. Thiscould significantly enhance the ability to study the roles of proteins in many different cellularreactions. There are some organic fluorophores that can be monovalently linked to proteins.Unfortunately, they are not photostable enough to be used for many biological studies. Quantumdots have significantly greater brightness and photostability. However, they aggregate and aremultivalent in their linkages. This project will optimize the imaging techniques and designs ofquantum dots to reduce aggregation and ensure that their linkages to proteins are monovalent.