Comparative cDNA Sequencing in Radish (Raphanus), a Crop, Weed, and Model System in Ecology and Evolution

萝卜属植物有两种：萝卜是食用萝卜，萝卜是野生萝卜。野生萝卜之所以重要，是因为它可能是萝卜的祖先，是世界范围内日益严重的作物杂草，也因为野生萝卜和作物萝卜的杂交已经成为加利福尼亚州自然土地上的入侵物种。萝卜也是生态学和进化论的模式系统，特别是在动植物相互作用和自然选择的研究中。尽管萝卜作为农作物、杂草和研究物种很重要，但几乎没有可用的基因序列数据，这严重限制了未来对萝卜生物学的理解进展。该项目将从作物和野生萝卜物种中产生数千个基因序列，并产生数百个遗传标记。这些序列和标记将使下一代发现成为可能，包括：作物驯化、杂草和入侵物种进化、对传粉者的适应、草食动物和全球变化的遗传基础。将产生的基因序列与同一科其他物种(作物属油菜和模式植物拟南芥)的现有序列进行比较，将有助于我们理解基因序列是如何进化的。获得项目成果产生的序列、标记和克隆将通过基因库、拟南芥生物资源中心(ABRC：http://www.biosci.ohio-state.edu/pcmb/Facilities/abrc/abrchome.htm)，植物基因组数据库(PlantGDB：http://www.biosci.ohio-state.edu/pcmb/Facilities/abrc/abrchome.htm)，))免费提供给更广泛的研究社区拟南芥信息资源(TIR：http://www.arabidopsis.org/))和基因组研究所的网站(TIGR：http://www.tigr.org/)和密歇根州立大学(密歇根州立大学：http://www.kbs.msu.edu/Faculty/Conner/Index.htm).密歇根州立大学的网站将以协作内容创建(Wiki)为特色，以促进全球萝卜研究社区之间的想法和数据交流。

There are two species in the plant genus Raphanus: R. sativus is the edible radish and R. raphanistrum is the wild species. The wild species is important because it is the likely ancestor of the crop, it is an increasingly serious crop weed worldwide, and because hybrids between the wild and crop radish have become an invasive species of natural land in California. Radish is also a model system in ecology and evolution, especially for studies of plant-animal interactions and natural selection. Despite its importance as a crop, weed, and study species, almost no gene sequence data is available for radish, severely limiting future progress in understanding the biology of radish. This project will produce sequences of thousands of genes from both the crop and wild radish species and generate hundreds of genetic markers. These sequences and markers will enable the next generation of discoveries, including the genetic basis of: crop domestication, weed and invasive species evolution, adaptation to pollinators, herbivores, and global change. Comparisons of the gene sequences generated to existing sequences from other species in the same family (the crop genus Brassica and the model plant Arabidopsis) will advance our understanding of how gene sequences evolve. Access to project outcomesThe sequences, markers, and clones produced will be made freely available to the broader research community via GenBank, the Arabidopsis Biological Resource Center (ABRC: http://www.biosci.ohio-state.edu/pcmb/Facilities/abrc/abrchome.htm), Plant Genome Database (PlantGDB: http://www.biosci.ohio-state.edu/pcmb/Facilities/abrc/abrchome.htm), the Arabidopsis Information Resource (TAIR: http://www.arabidopsis.org/) and websites at the Institute for Genomic Research (TIGR: http://www.tigr.org/) and Michigan State University (MSU: http://www.kbs.msu.edu/Faculty/Conner/Index.htm). The MSU website will feature collaborative content creation (wiki) to facilitate the exchange of ideas and data among the worldwide radish research community.