Basophils as modulators of humoral and cellular allo-immune responses

我们已经证明，在记忆免疫反应中，嗜碱性粒细胞能够与其表面大量完整的抗原结合。这种结合是由抗原特异性的IgE分子和嗜碱性粒细胞表面高亲和力的IgE受体介导的。在再次暴露于抗原后，嗜碱性粒细胞被激活，并构成脾和骨髓中IL-4和IL-6的主要来源。激活的嗜碱性粒细胞显著改变了CD4+T细胞的表型，并在免疫和感染模型中显示出在记忆免疫反应的发展中发挥重要作用。记忆型免疫反应在移植中占主导地位，尤其是在急性排斥反应或慢性移植排斥反应的背景下。我们假设嗜碱性粒细胞在急性和慢性移植排斥反应中作为体液和细胞免疫反应的调节器发挥了重要作用，并且嗜碱性粒细胞的激活是正在进行的记忆免疫反应的良好指标。这些方面将在体外使用MLR反应、在移植的小鼠模型中以及在移植后的患者中进行研究。

We have shown that during memory immune responses basophils are able to bind significant amounts of intact antigens on their surface. This binding is mediated by antigen-specific IgE molecules and high affinity IgE receptors on basophils. After re-exposure to antigens basophils become activated and constitute the main source of IL-4 and IL-6 in the spleen and bone marrow. Activated basophils markedly alter the phenotype of CD4+ T cells and play an important role in the development of memory immune responses as shown in models of immunization and infection. A memory type immune response predominates in transplantation, especially after acute rejections or in the setting of chronic transplant rejection. We hypothesize that basophils play an important role as modulators of humoral and cellular immune responses during acute and chronic transplant rejection and that activation of basophils is a good indicator for ongoing memory immune responses. These aspects will be investigated in vitro using MLR reactions, in mouse models of transplantation, and in patients after transplantation.