Functional analysis of the histone arginine methyltransferase PRMT6 (A03)

蛋白精氨酸甲基转移酶6 （PRMT6）催化组蛋白H3在精氨酸2 （H3R2me2a）上的不对称二甲基化，这有助于根据其基因组位置、增强子激活和启动子抑制而产生相反的转录效应。在下一个资助期内，我们将描述H3R2me2a与增强子标记的串音，PRMT6与染色质的结合位点和募集机制，其靶位点特异性染色质相互作用物，以及PRMT6/H3R2me2a在神经发生中的体内功能。

The Protein arginine methyltransferase 6 (PRMT6) catalyzes asymmetric dimethylation of histone H3 at arginine 2 (H3R2me2a), which contributes to opposing transcriptional effects depending on its genomic location, activation at enhancers and repression at promoters. In the next funding period we will characterize the cross-talk of H3R2me2a with enhancer marks, the binding sites and recruitment mechanisms of PRMT6 to chromatin, its target site-specific chromatin interactors and finally the in vivo function of PRMT6/H3R2me2a in neurogenesis.