SGER: Carbon Nanomaterial Translocation and Transfer

在过去的四年里，PI研究了RNA和单壁碳纳米管（SWNTs）的结合，并首次证明了单壁碳纳米管的RNA递送。PI发明了溶血磷脂作为溶解swnt的一种非常有效的方法。利用分子动力学模拟和透射电子显微镜，PI和他的团队揭示了溶血磷脂与SWNT的结合主要沿着管轴排列。PI和他的合作者还发现了水生模型系统大水蚤对脂质包裹的SWNTs的体内修饰。为了获得纳米毒性的机制理解，PI和他的合作者模拟了富勒烯C60的准备摄取和富勒烯衍生物C60(OH)20在DPPC双分子层上的最小易位。这些开创性的研究研究了生物和环境系统中的碳纳米材料，并促进了本提案的以下三个目标。1)了解富勒烯C70与苯酚没食子酸的结合方式及其生物相容性。2)实时检测c70 -没食子酸的细胞易位。3)研究碳纳米材料在食物链中的转移。

SGER: Carbon Nanomaterial Translocation and TransferOver the past four years the PI has studied the binding of RNA and single-walled carbon nanotubes (SWNTs) and first demonstrated RNA delivery by SWNTs. The PI has invented the use of lysophospholipids as a remarkably efficient method for solubilizing SWNTs.Using molecular dynamics simulations and transmission electron microscopy the PI and his team have unraveled that the binding of lysophospholipids to an SWNT as predominantly aligned along the tube axis. The PI and his collaborators have also discovered the in vivo modification of lipid-coated SWNTs by aquatic model system Daphnia magna. Towards obtaining a mechanistic understanding of nanotoxicity, the PI and his collaborators have simulated the ready uptake of fullerene C60 and the minimal translocation of fullerene derivative C60(OH)20 across a DPPC bilayer. These pioneering studies have examined carbon nanomaterials in biological and environmental systems and have facilitated the following three objectives of this proposal. 1) Understand the binding mode of fullerene C70 and phenol gallic acid for biocompatibility. 2) Detect the cellular translocation of C70-gallic acid in real time. 3) Investigate the transfer of carbon nanomaterials in the food chain.