Multiple biomarker sensor for early detection of ovarian cancer

本研究的目的是研制一种多生物标志物检测传感器，用于卵巢癌的早期检测和监测。该方法基于声表面波(SAW)换能器，该换能器在单个设备上集成了两个关键功能，即去除干扰蛋白质和检测多个生物标志物。技术优势该项目首次提出并演示了使用锯子去除传感器表面的非特异性结合(NSB)蛋白。NSB是利用任何传感原理、光学、电化学或质量变化的传感器响应的混杂因素。所提出的传感器是第一个将这种移除功能与任何设备的传感功能相结合的传感器。这是首次尝试差异去除结合蛋白，一次一个，以实现对单个设备表面上多个部分的检测和定量。许多疾病，如卵巢癌，都以体液中特定蛋白质水平升高为特征。因此，该传感器在医学诊断中具有广泛的应用前景。更广泛的影响这项工作将允许比目前的做法更广泛和更容易的测试，也适用于环境和化学检测应用的多个部分的检测。拟议的跨学科高级设计项目的试点测试可以为工程教育带来一个可持续的模式。建议的实际操作传感器单元和相关实验将使参与的学童了解传感器的基本原理和概念。拟议的少数族裔辅导计划可能会导致招募到科学和工程项目。

ObjectiveThe objective of this research is to develop a sensor for the detection of multiple biomarkers with specific application to early detection and monitoring of ovarian cancer. The approach is based on a surface acoustic wave (SAW) transducer that integrates two critical functions, namely, removal of interfering proteins, and sensing of multiple biomarkers, on a single device. Technical MeritThis project is the first to propose and demonstrate removal of non-specifically bound (NSB) proteins from sensor surfaces using SAWs. NSB is a confounding factor for sensor response utilizing any sensing principle, optical, electrochemical or mass change. The proposed sensor is the first to integrate this removal function with the sensing function of any device. This is the first attempt at differential removal of bound proteins, one at a time, to achieve detection and quantification of multiple moieties on a single device surface. Many diseases, such as ovarian cancer, are characterized by elevated levels of select proteins in bodily fluids. Hence, this sensor will have wide applicability in medical diagnostics. Broader ImpactThis work will allow for more wide-spread and easy testing than current practice and is also applicable to detection of multiple moieties for environmental and chemical detection applications. The proposed pilot testing of an interdisciplinary senior design project can lead to a sustainable model for engineering education. Proposed hands-on sensor units and associated experiments will lead to awareness of basic sensor principles and concepts among the participating school children. The proposed minority mentoring program can lead to recruitment into science and engineering programs.