Permanent sterile Oberflächen durch Kombination von antimikrobiellen und Biobelag verhindernden Oberflächenstrukturen

为了克服当今抗菌材料的不足，该项目的目的是开发长期无菌的聚合物表面，同时抗菌和抗生物污染。虽然抗菌组分将杀死病原体，但抗生物污染组分将通过防止蛋白质、细胞或细胞碎片的不可逆附着并因此防止生物膜的形成而使表面自清洁。为了制造这样的表面，我们需要彻底了解抗菌聚合物表面如何杀死细菌细胞，以及抗菌聚合物如何防止粘附。因此，我们需要知道每个表面参数（化学成分，阳离子类型和电荷密度，疏水性，补丁大小，层厚度，接枝密度，粗糙度，润湿性，溶胀性等）和每个环境参数（pH、离子强度、缓冲液组成、温度等）影响细胞表面的相互作用。因此，除了获得完全无菌表面的长期目标外，该项目的目的是增加对聚合物表面-细胞相互作用的基本理解。我们遵循这些方法：（1）我们将确定不与磷脂，脂多糖，肽聚糖和其他细胞碎片形成不可逆复合物的抗菌聚电解质，并将其用作抗菌成分，以及（2）我们将从混合的抗菌-抗生物污染聚合物合成纳米结构表面。如果时间允许，我们将把抗菌聚合物整合到分层的、可酶降解的、自我再生的表面上。

To overcome the deficiencies of today's antibacterial materials, the aim of this project is to develop long-term sterile polymer surfaces that are simultaneously antibacterial and antibiofouling. While the antibacterial component will kill pathogens, the antibiofouling component will make the surface self-cleaning by preventing irreversible attachment of proteins, cells or cell debris, and thus the formation of a biofilm. To make such surfaces, we need a thorough understanding of how antimicrobial polymer surfaces kill bacterial cells, and how antibiofouling polymers prevent adhesion. Thus, we need to know how each surface parameter (chemical composition, cation type and charge density, hydrophobicity, patch size, layer thickness, grafting density, roughness, wettability, swellability etc.) and each environmental parameter (pH, ionic strength, buffer composition, temperature etc.) influences the surface-cell interaction. Therefore, besides the long-term aim to obtain the perfectly sterile surface, the aim of this project is to increase the fundamental understanding of polymer surface-cell interactions. We follow these approaches: (1) We will identify antimicrobial polyelectrolytes that do not form irreversible complexes with phospholipids, lipopolysaccharide, peptidoglycan, and other cell debris, and use them as the antibacterial component, and (2) We will synthesize nanostructured surfaces from mixed antimicrobial-antibiofouling polymers. Time permitting, we will integrate antimicrobial polymers into layered, enzymatically degradable, self-regenerating surfaces.