Purchase of a Research Grade MALDI-ToF Mass Spectrometer

根据化学研究仪器和设施多用户计划（CRIF:MU）的这份合同，约翰霍普金斯大学的John P. Toscano教授将获得一个矩阵辅助激光解吸/电离飞行时间（MALDI-ToF）质谱仪，该质谱仪将被安置在约翰霍普金斯大学霍姆伍德校区化学系的质谱仪设施中。该仪器将为来自5个不同院系（化学、生物、材料科学与工程、化学与生物分子工程、地理与环境工程）的23个用户以及附近机构（摩根州立大学、陶森大学和洛约拉学院）的额外用户提供常规和专业分析质谱服务。化学系的主要用户D. P. Goldberg， M. M. Greenberg， J. P. Roth， J. P. Toscano， J. D. Tovar和C. A. Townsend将使用该光谱仪研究腐蚀酶和DNA损伤和修复，表征含血红素加氧酶和过氧化物酶中的氧化氨基酸修饰，以及hno诱导的涉及心脏信号通路的蛋白质修饰。分子电子材料和有机共轭聚合物的表征以及模块化和迭代生物合成酶中共价中间体的检测和表征。maldi - tof质谱法是获得非常大分子精确分子量的首选技术，具有极高的灵敏度。因此，使用MALDI-ToF质谱仪已成为一种标准技术，特别是在涉及生物分子的研究中。这项研究将在许多领域产生重大影响，特别是化学和生物化学。该仪器也将成为教学和研究的一个组成部分。

With this award from the Chemistry Research Instrumentation and Facilities Multiuser Program (CRIF:MU), Professor John P. Toscano from John Hopkins University will acquire a matrix assisted laser desorption/ionization time of flight (MALDI-ToF) mass spectrometer that will be housed in the Department of Chemistry's Mass Spectrometry Facility on the Homewood campus of Johns Hopkins University. The instrument will provide both routine and specialized analytical mass spectral services to about 23 total users, from five different departments (Chemistry, Biology, Materials Science and Engineering, Chemical and Biomolecular Engineering, and Geography and Environmental Engineering) as well as additional users from institutions within the immediate area (Morgan State University, Towson University, and Loyola College). The major users from the Chemistry Department, D. P. Goldberg, M. M. Greenberg, J. P. Roth, J. P. Toscano, J. D. Tovar, and C. A. Townsend will use this spectrometer for studies of corrolazines and DNA damage and repair, characterization of oxidative amino acid modifications in heme-containing oxygenases and peroxidases and HNO-induced modification of proteins involved in cardiac signaling pathways, characterization of molecular electronic materials and organic conjugated polymers and detection and characterization of covalent intermediates in modular and iterative biosynthetic enzymes.MALDI-ToF-mass spectrometry is the technique of choice for obtaining accurate molecular weights of very large molecules, with extremely high sensitivity. Use of a MALDI-ToF mass spectrometer has therefore become a standard technique, especially in studies involving biomolecules. This research will have a significant impact in a number of areas, especially chemistry and biochemistry. This instrument will also be an integral part of teaching as well as research.