Arabidopsis 2010: Regulation of the proembryo boundary in Arabidopsis

沃尔夫冈·卢科维茨建议编号IOS-0842284拟南芥原胚边界的调节植物身体由两组干细胞产生，根和茎顶端分生组织。在很大程度上，人们仍不清楚这些干细胞利基是如何在早期胚胎中首次建立的。这项拟议的研究将分析HAN在定位根干细胞生态位中的作用，HAN是一种预测的基因活性调节因子。初步工作，主要是通过遗传学和分子生物学的方法，揭示了HAN决定了两种不同细胞类型之间的指导性边界的位置，称为“原胚胎边界”。跨越这一边界的信号是在胚胎只有8个细胞时建立的，随后组织根干细胞生态位。将遵循一种综合的方法，包括标准的遗传和分子生物学技术、基因组学、显微镜和生物化学，目的是从机械上理解原胚胎边界是如何建立和调节的。重点将着重分析汉族在这一过程中的作用以及与汉族密切相关的类汉因素。预期的结果将对我们对植物模式形成过程的基本理解做出重大而新颖的贡献，并可能为在培养中启动根干细胞生态位的有针对性的方法提供信息。该项目将导致植物遗传学、分子生物学、基因组学和高级显微镜方面的博士后研究员和研究生的培训。高中生和本科生的参与是研究计划不可或缺的一部分。学生将通过植物生物系的计划，以及大学的暑期本科生研究计划(SURP)和由NSF赞助的桃州路易斯·斯托克斯少数民族参与联盟(PSLSAMP)进行招生。

Wolfgang LukowitzProposal number IOS-0842284Arabidopsis 2010: Regulation of the proembryo boundary in ArabidopsisThe plant body is produced by two groups of stem cell, the root and shoot apical meristems. It remains largely unknown how these stem cell niches are first established in the early embryo. The proposed research will analyze the role of HAN, a predicted regulator of gene activity, in positioning the root stem cell niche. Preliminary work, largely by the methods of genetics and molecular biology, reveals that HAN determines the position of an instructive boundary between two different cell types, termed the "proembryo boundary". Signaling across this boundary, which is set up when the embryo has only eight cells, subsequently organizes the root stem cell niche.A comprehensive approach, including standard genetic and molecular biology techniques, genomics, microscopy, and biochemistry, will be followed with the aim to mechanistically understand how the proembryo boundary is established and regulated. Special emphasis will be placed on analyzing the role of HAN and closely related HAN-like factors this process. The expected results will make a significant and novel contribution to our fundamental understanding of patterning processes in plants and possibly inform targeted approaches for initiating root stem cell niches in culture.The project will lead to the training of a postdoctoral fellow and a graduate student in plant genetics, molecular biology, genomics, and advanced microscopy. Participation of high school and undergraduate students is an integral part of the research plan. Students will be recruited through programs of the Plant Biology Department, as well the University's Summer Undergraduate Research Program (SURP) and the NSF-sponsored Peach State Louis Stokes Alliance for Minority Participation (PSLSAMP).