MRI: Acquisition of Next Generation DNA Sequencing Equipment

这项重大研究仪器(MRI)奖资助达特茅斯学院购买基因组分析仪IIx测序仪，以实现深度测序，这是生物和医学领域的一项新技术。深度测序技术使到目前为止几乎不可能进行的实验成为可能，并为研究人员提供了一种方法，可以在给定的时间确定细胞中所有DNA和RNA的准确序列。了解细胞在正常和疾病状态下的功能和与环境的相互作用是所有生物和医学研究人员的主要目标。达特茅斯的Illumina Genome Analyzer IIx将使研究人员能够(I)精确定位与酶反应、微生物生物膜、植物生理学和许多其他生物现象相关的基因突变和变异；(Ii)确定参与基因调控的特定DNA结合蛋白结合的基因组位置；以及(Iii)识别和量化作为环境、毒理和生理反应的函数的mRNA表达的差异。这些研究的结果将发表在同行评议的期刊上，并存放在公共数据库中，以造福于生物领域。此外，通过他们的研究活动，他们对达特茅斯REU网站(www.dartmailth.edu/~surf)的赞助，以及他们对个别学生的指导，私人投资和共同投资都深深地致力于培训。此外，所有的私人投资机构和合作的个人投资机构通过K-12学校和/或佛蒙特州诺里奇市的蒙特郡科学博物馆的外展项目传播科学知识。这些活动鼓励更广泛地参与科学，促进培养和发展下一代研究科学家。

This Major Research Instrumentation (MRI) award funds the acquisition of a Genome Analyzer IIx sequencer to enable deep sequencing, a new technology in biological and medical fields, at Dartmouth College. Deep sequencing technology enables experiments that were, until now, virtually impossible to perform, and provides a way for the researcher to determine the exact sequence of all the DNA and RNAs in a cell at a given time. Understanding how cells function and interact with their environment in normal and disease states is the major goal of all biological and medical researchers. The Illumina Genome Analyzer IIx at Dartmouth would enable researchers to (i) pinpoint genetic mutations and variations associated with enzymatic reactions, microbial biofilms, plant physiology, and many other biological phenomena; (ii) determine the genomic locations bound by specific DNA binding proteins involved in gene regulation; and (iii) identify and quantify differences in mRNA expression as a function of environmental, toxicological, and physiological responses. The results from these studies would be published in peer-reviewed journals and deposited in public databases for the benefit of the biological fields. Furthermore, through their research activities, their sponsorship of the Dartmouth REU-SITE(www.dartmouth.edu/~surf), and their mentorship of individual students, the PIs and the co-PIs are deeply committed to training. Additionally, all of the PIs and co-PIs disseminate scientific knowledge via outreach programs in K-12 schools and/or the Montshire Science Museum in Norwich, Vermont. These activities encourage broader participation in science and promote the training and development of the next generation of research scientists.