MRI: Acquisition of a tandem (IT-TOF) mass spectrometer system for biological research and application

0923027 san本提案的目标是为生物研究和教育建立一个中央质谱分析设施。为了实现这一目标，已经组建了一个多学科研究小组。这些团队成员中有几个拥有相同的研究兴趣，并且团队之间已经存在协同合作/互动。该设施将支持广泛的研究活动，跨越五个学术部门，包括：生物工程，化学和生物分子工程，土木和环境工程，生物化学和细胞生物学，以及化学。该设施将为若干生物项目提供急需的定量和分析仪器。他们建议为该设施配备离子阱飞行时间（2D-nano-LC-IT-TOF）质谱仪。此外，质谱仪将与二维纳米lc系统接口，以进一步提高他们的分析能力。本提案所要求的仪器将为许多生物、化学或工程领域的项目提供必要的定量和分析工具。这些项目将有助于更好地了解生物特性和功能，更好地设计用于合成生物制品的生物催化剂，以及更好的生物修复策略。此外，该设施还将成为促进和加强多学科互动以及产生新想法和合作项目的有效工具。这些项目将为研究生和本科生以及博士后研究人员提供培训机会。参与这类研究项目的学生将受益于生物化学和代谢工程、生物系统工程、生物化学和分子生物学等高度跨学科的培训。此外，将开发与现有课程相结合的新实验模块。与过去一样，这些实验室将继续为许多妇女和少数民族研究生、本科生和高中生提供培训机会。

0923027SanThe goal of this proposal is to establish a central mass spectrometry facility for biological research and education. To achieve this goal, a multidisciplinary research team has been assembled. Several of these team members share the same research interests and synergistic collaborations/interactions among the group already exist. The facility will support a wide range of research activities, spanning across five academic departments which include: Bioengineering, Chemical and Biomolecular Engineering, Civil and Environmental Engineering, Biochemistry and Cell Biology, and Chemistry. The facility will provide much needed quantitative and analytical instrumentation for a number of biological projects. They propose to equip the facility with an ion trap time of flight (2D-nano-LC-IT-TOF) mass spectrometer. In addition, the mass spectrometer will be interfaced with a 2-D nano-LC system to further enhance their analytical capability. The instrumentation requested in this proposal will provide the necessary quantitative and analytical tools for many projects at the interface of biology and chemistry or engineering. These projects will result in a better understanding of biological properties and functions, better design of biocatalysts for the synthesis of bioproducts, and better strategies for bioremediation. In addition, the facility will also serve as an effective vehicle to foster and strengthen multidisciplinary interactions and generate new ideas and collaborative projects. The projects will provide training opportunities for graduate and undergraduate students and postdoctoral research associates. Students participating in these types of research projects will benefit from highly interdisciplinary training in biochemical and metabolic engineering, biosystems engineering, biochemistry and molecular biology. In addition, new laboratory modules, which will be integrated into existing courses, will be developed. As in the past, the laboratories will continue to provide training opportunities for many women and minority graduate, undergraduate and high school students.