MRI: Acquisition of a Liquid Chromatograph-Tandem Quadrupole-Orbitrap Fourier Transform Mass Spectrometer for High-Throughput Biological Analyses

MRI：获取用于高通量生物分析的液相色谱串联四极杆轨道阱傅里叶变换质谱仪

• 批准号: 1530975
• 负责人: Shawn Campagna
• 金额: $ 41.24万
• 依托单位: University of Tennessee Knoxville
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1530975&HistoricalAwards=false
• 关键词: MRI; Acquisition; Liquid; Chromatograph; Tandem

An award is made to the University of Tennessee (UT) to acquire a Dionex UltiMate 3000 liquid chromatograph-Q Exactive Plus tandem quadrupole-Orbitrap Fourier transform mass spectrometer (LC Q Exactive), which will be the center piece instrument of the institution's Biological and Small Molecule Mass Spectrometry Core (BSMMSC) that provides state-of-the-art metabolomics and lipidomics support. This instrument will have broad impacts for the training and outreach capabilities or the institution and core, and acquisition of the LC-Q Exactive will strengthen both intra- and inter-organizational collaborations by providing new capabilities and infrastructure. Further, this instrument will also have impacts for UT's teaching mission as it will be used to train a diverse pool of post-doctoral, graduate, and undergraduate researchers. At the undergraduate level, introduction to meritorious research in the emerging fields of metabolomics and biological mass spectrometry will not only be accomplished through independent research courses but also through summer undergraduate programs and classroom exercises. The instrument will be made available to enhance two NSF Research Experience for Undergraduate (REU) programs in the departments of Microbiology and Chemistry at UT Knoxville; and the PI and Co- PIs are also working to develop a new co-listed Nutrition and Chemistry course that will utilize the LC-Q Exactive. At the graduate level, the instrument will be made available for research, and users will receive training on all aspects of experimental design and execution. Further, the LC-Q Exactive will be made available to graduate courses given by the Chemistry, Nutrition, and Biochemistry and Cellular and Molecular Biology departments to enhance instruction in these classes. The PI and Co-PIs will make these resources available to help broaden participation in the STEM disciplines and will collaborate with Mr. Travis Griffin and the NSF Funded Tennessee Louis Stokes Association for Minority Participation (TLSAMP) program to identify undergraduate student participants from underrepresented groups and with Dr. Laurie Richardson at Florida International University (~75% of students from this institution are from underrepresented groups.) to engage both undergraduate and graduate students.Systems Biology technologies are important drivers for biological investigation and discovery,and these tools fall into three broad categories: genomics, proteomics, and metabolomics. The systems biology hierarchy aims to measure genes and RNA transcripts, protein content, and metabolic activity, respectively. While genomics and proteomics platforms are well established, metabolomics and (and to some extent lipidomics) platform development is still in its infancy. Significant infrastructure for systems biology is needed at UT to ensure that approaches which integrate large scale measures of transcripts, proteins and metabolites to develop holistic models of cellular, tissue and organismal function are available to the University community and others in the region. The LC-Q Exactive system will allow the development of novel techniques for discovery metabolomics and lipidomics and will provide the BSMMSC with basic targeted proteomics capabilities that will bolster available resources for bioanalytical science and advance capabilities in the region. A number of researchers within or near the UT Knoxville campus will benefit from these capabilities to measure metabolites, lipids, and proteins that can be applied to the study of all kingdoms of life. This instrument will enable research in a broad range of scientific fields ranging including, but not limited to, marine environmental science, soil microbiology, eukaryotic and prokaryotic biochemistry, computer science, and analytical chemistry.

田纳西大学(UT)获得了Dionex Ultra3000液体色谱仪-Q Exactive Plus串联四极轨道傅立叶变换质谱仪(LC Q Exactive)，这将是该机构提供最先进的代谢组学和脂类组学支持的生物和小分子质谱学核心(BSMMSC)的核心仪器。这一工具将对培训和外联能力或机构和核心产生广泛影响，收购LC-Q Exactive将通过提供新的能力和基础设施加强组织内和组织间的合作。此外，该仪器还将对德克萨斯大学的教学任务产生影响，因为它将用于培养不同的博士后、研究生和本科生研究人员。在本科阶段，介绍代谢组学和生物质谱学新兴领域的有价值的研究不仅将通过独立的研究课程完成，还将通过暑期本科课程和课堂练习来完成。该仪器将用于加强德克萨斯州诺克斯维尔大学微生物学和化学系的两个NSF本科生研究经验(REU)项目；PI和Co-Pis还在努力开发一门新的联合列出的营养和化学课程，该课程将利用LC-Q Exactive。在研究生阶段，该仪器将用于研究，用户将接受关于实验设计和执行的所有方面的培训。此外，化学、营养和生物化学以及细胞和分子生物学系的研究生课程将提供LC-Q Exactive，以加强这些课程的教学。PI和Co-PIs将提供这些资源，以帮助扩大STEM学科的参与，并将与Travis Griffin先生和NSF资助的田纳西州路易斯·斯托克斯少数族裔参与协会(TLSAMP)计划合作，从代表性不足的群体中确定本科生参与者，并与佛罗里达国际大学的Laurie Richardson博士(该机构约75%的学生来自代表性不足的群体)合作。系统生物学技术是生物学研究和发现的重要驱动力，这些工具分为三大类：基因组学、蛋白质组学和新陈代谢组学。系统生物学层次结构旨在分别测量基因和RNA转录本、蛋白质含量和代谢活性。虽然基因组学和蛋白质组学平台已经很好地建立了，但代谢组学和(在某种程度上是脂质组学)平台的开发仍处于初级阶段。德克萨斯大学需要系统生物学的重要基础设施，以确保大学社区和该地区的其他人可以使用整合大规模转录本、蛋白质和代谢物测量的方法，以开发细胞、组织和组织功能的整体模型。LC-Q Exactive系统将允许开发发现代谢组学和脂类组学的新技术，并将为BSMMSC提供基本的有针对性的蛋白质组学能力，这将加强该地区生物分析科学和先进能力的现有资源。德克萨斯大学诺克斯维尔校区内或附近的许多研究人员将受益于这些能力，以测量可应用于所有生命王国研究的代谢物、血脂和蛋白质。该仪器将使广泛的科学领域的研究成为可能，包括但不限于海洋环境科学、土壤微生物学、真核生物和原核生物生物化学、计算机科学和分析化学。