Genomic dissection of floral transition in Brassica napus towards crop improvement by life cycle adaptation and hybrid yield increase

甘蓝型油菜（Brassica napus L.）由于该物种的历史较短，遗传变异较少。育种者试图通过从世界其他地理区域的非适应材料中进行基因渗入来扩大遗传基础。然而，这些材料的使用尤其受到非适应开花时间（FTi）的阻碍。在这里，提出了一个综合的项目，以获得更深入的了解FTi的全球表达分析和克隆的主要FTi调节。候选基因将通过重组作图，并与其他小组合作，通过关联作图进行作图。作为概念研究的证明，相关序列将被定位到携带外来油菜籽渐渗的重组系。第二部分研究4个FTi基因与杂种优势的相关性。假设这些基因内的序列变异将对种子产量和生物量杂种优势产生影响，将通过TILLING鉴定突变体。在第二个资助期，将对突变体进行分析，并进行杂交，以确定F1杂种的杂种优势。

Rapeseed (Brassica napus L.) suffers from low genetic variation due to the short history of this species. Breeders try to broaden the genetic basis by gene introgression from non-adapted material from other geographic regions of the world. However, use of these materials is hampered, among others, by non-adapted flowering time (FTi). Here an integrated project is proposed to get a deeper understanding of FTi by global expression analysis and cloning of major FTi regulators. Candidate genes will be mapped by recombination mapping and, in collaboration with other groups, by association mapping. As a proof of concept study, relevant sequences will be mapped to recombinant lines carrying exotic rapeseed introgressions. The 2nd part of the project will study the relevance of 4 FTi genes for heterosis. Assuming that sequence variation within these genes will have an impact on seed yield and biomass heterosis, mutants will be identified by TILLING. The mutants will be analyzed and crosses will be made to determine heterosis of F1 hybrids in the 2nd funding period.