Genomic dissection of floral transition in Brassica napus towards crop improvement by life cycle adaptation and hybrid yield increase

油菜(Brassica napus L.)由于本种的历史较短，遗传变异较低。育种者试图通过从世界其他地理区域的非适应材料中导入基因来拓宽遗传基础。然而，这些材料的使用受到非适应开花时间(FTI)等因素的阻碍。在这里，提出了一个综合项目，通过全球表达分析和克隆主要的FTI调控因子来更深入地了解FTI。候选基因将通过重组作图和与其他群体合作的关联作图来定位。作为概念研究的证明，相关序列将被映射到携带外来油菜籽导入的重组系。该项目的第二部分将研究4个FTI基因与杂种优势的相关性。假设这些基因内的序列变异将对种子产量和生物量杂种优势产生影响，将通过耕作来鉴定突变体。将对突变体进行分析，并进行杂交，以确定第二个资助期的F1杂种优势。

Rapeseed (Brassica napus L.) suffers from low genetic variation due to the short history of this species. Breeders try to broaden the genetic basis by gene introgression from non-adapted material from other geographic regions of the world. However, use of these materials is hampered, among others, by non-adapted flowering time (FTi). Here an integrated project is proposed to get a deeper understanding of FTi by global expression analysis and cloning of major FTi regulators. Candidate genes will be mapped by recombination mapping and, in collaboration with other groups, by association mapping. As a proof of concept study, relevant sequences will be mapped to recombinant lines carrying exotic rapeseed introgressions. The 2nd part of the project will study the relevance of 4 FTi genes for heterosis. Assuming that sequence variation within these genes will have an impact on seed yield and biomass heterosis, mutants will be identified by TILLING. The mutants will be analyzed and crosses will be made to determine heterosis of F1 hybrids in the 2nd funding period.