Metabolic determinants in the interaction of biotrophic and hemibiotrophic fungi with cereals

项目B6的主要目标是阐明真菌效应子调控植物初级代谢的分子决定因素。此外，将在条件性dsRNAi大麦敲低系（B3）和玉米转化体（A2，B3）中研究相容植物/病原体相互作用期间所选代谢过程的相关性。为了深入了解植物-微生物互作过程中诱导的代谢和分子变化，将在生理、代谢和转录水平上研究玉米黑粉菌（Ustilago maydis）、小麦白粉病菌（Blumeria graminis）、辣椒白粉病菌（Erysiphe cichoracearum）、禾生炭疽病菌（Colletotrichum graminicola）和印度梨形孢菌（Piriformospora indica）与各自宿主的互作。收集的数据将在中央数据库中保存和管理（由B7协调的中央实验）。随后的数据挖掘将允许识别参与相容的植物-微生物相互作用的关键代谢物和转录物。这些发现的意义将在候选基因（B3）表达改变的转基因植物和随后的感染研究中进行测试。为了深入了解成功入侵植物所需的可能的真菌决定因素，美国。不能成功感染其宿主植物的玉米突变体（A2）将被包括在这些研究中。

Major objective of project B6 is to unravel molecular determinants underlying the regulation of plant primary metabolism by fungal effectors. In addition the relevance of selected metabolic processes during compatible plant/pathogen interaction will be investigated in conditional dsRNAi barley knock-down lines (B3) and maize transformants (A2, B3). To gain insight into metabolic and molecular changes induced during plant-microbe interaction, interactions of Ustilago maydis, Blumeria graminis, Erysiphe cichoracearum, Colletotrichum graminicola and Piriformospora indica with their respective hosts will be studied on the physiological, metabolic and transcriptional level. Data collected will be deposited and managed in a central database (central experiment coordinated by B7). Subsequent data mining will allow identifying key-metabolites and transcripts involved in compatible plant-microbe interactions. The significance of these findings will be tested in transgenic plants altered in expression of candidate genes (B3) and in subsequent infection studies. To gain insight into possible fungal determinants required for the successful invasion of plants, U. maydis mutants (A2), unable to successfully infect their host plants, will be included in these studies.