RUI: Mechanism of Antibiotic Resistance Associated with the Arabidopsis ABC Protein, Atwbc19

智力优势ABC（ATP结合盒）蛋白代表了一个基因家族，该家族在植物中得到了极大的扩展，在拟南芥和水稻中的成员数量（超过120个）是任何其他多细胞生物体（例如，拟南芥和水稻）测序基因组的两倍。人类、蠕虫、苍蝇）。这个家族在植物中扩展的原因尚不清楚，但暗示了王国特定的进化限制。 该项目的重点是拟南芥ABC蛋白，Atwbc19（ABCG19），一个植物特异性分支的成员。 Atwbc19赋予抗生素耐药性，但其潜在机制尚不清楚。初步结果表明，Atwbc19不作为一个转运，但作为一个翻译调节。因此，该项目的目标是证明Atwbc 19的切割产生可溶性ABC蛋白，确认其与翻译装置的关联并鉴定受调节的转录本。最终，可能会建立一个新的植物抗生素耐药性范例。更广泛的影响该项目有三个教育目标。首先，将为本科生提供长期研究机会。 第二，将开发一个基于研究的课程。最后，研究经验将在传统的课堂上通过上层植物生物学课程提供。总的来说，该项目将有助于学院的战略目标，增加学生参与研究或实习从30%到2015年100%。在部门一级，该项目将有助于正在进行重大修订的课程，以更加强调将研究与教学联系起来。最终，一个目标是激励非裔美国女学生从事智力和个人发展，导致他们到研究生院。

INTELLECTUAL MERIT ABC (ATP binding cassette ) proteins represent a gene family that has greatly expanded in plants, with twice as many members in Arabidopsis and rice (over 120) as any other sequenced genome of a multicellular organism (eg. human, worm, fly). The reason behind the expansion of this family in plants is unknown but hints to kingdom specific evolutionary constraints. This project focuses on the Arabidopsis ABC protein, Atwbc19 (ABCG19), a member of a plant-specific clade. Atwbc19 confers antibiotic resistance but the underlying mechanism is not clear. Preliminary results suggest that Atwbc19 does not function as a transporter but as a translational regulator. Thus the goal of this project is to demonstrate cleavage of Atwbc19 resulting in a soluble ABC protein, confirm its association with the translation apparatus and identify regulated transcripts. Ultimately, a new paradigm for antibiotic resistance in plants may be established.BROADER IMPACT The project has three educational objectives. First, long term research opportunities will be provided to undergraduates. Second, a research based course will be developed. And finally, research experiences will be provided in the traditional classroom through an upper level Plant Biology course. Overall, the project will contribute to the strategic goal of the College to increase student participation in research or internships from 30% to 100% by 2015. At the departmental level, the project will contribute to a curriculum that is being significantly revised towards a stronger emphasis on linking research to teaching. Ultimately, one goal is to inspire African American female students to engage in intellectual and personal development that leads them to Graduate School.