Conference: Keystone Symposia meeting on "AAA+ and Related ATP-Driven Protein Machines" being held at the Granlibakken Resort, Tahoe City, CA

智力上的优点。 这个关于“AAA+和相关ATP驱动的蛋白质机器：结构，功能和机制”的关键研讨会的目标是刺激新概念和方法的发展，旨在深入了解一系列复杂细胞过程的分子机制。 会议重点关注需要由AAA+（与多种细胞活动相关的ATP酶）分子机器家族提供动力的机械工作的过程。 这些蛋白质组装体属于一类特殊的酶，其参与关键的生物过程，包括从DNA转录RNA、细胞分裂前的DNA复制、蛋白质的生物合成和周转、与膜从细胞的一部分运输到另一部分相关的高度调节的过程以及动态蛋白质支架或"细胞骨架"的重塑，"赋予细胞形状和移动性 本次会议将汇集具有非常不同的背景和观点的研究人员：物理科学家，他们正在将生物物理方法应用于复杂系统，生物学家，他们寻求对基本细胞过程的深入机械理解。 本次会议旨在通过弥合方法和学科界限来促进创新。 事实上，会议计划将汇集来自不同领域的演讲者，他们通常很少有机会互动，但阐明了不同AAA+蛋白质的关键方面。 演讲者还将包括那些在理解不属于AAA+家族但功能或结构相关的蛋白质方面取得突破的人。 这些观点超越了从细胞到原子水平的分子尺度，以及从细胞生物学和遗传学到单分子生物物理学的方法。 这种结合可能会导致对这些蛋白质的共同机制的深入了解，以及新的实验方法和发现。 更广泛的影响。 本次会议的构思和组织围绕着中心目标，以激发AAA+蛋白质研究的新想法和新方法。 为了实现这些目标，演讲者被选为许多不同生物学领域的先驱，或者在不同分析水平上取得了最新的概念或实验突破（例如，结构的、化学的、细胞的）。 这次会议将通过促进联网和协作来加强基础设施。 关于对受训人员和新参加的研究人员的教育益处，会议将实现以下目标：1）使这些人了解最新信息; 2）鼓励进行批判性的科学分析; 3）促进他们的职业发展，有机会参与讨论切割-边缘科学，并与可能有助于其科学和专业发展的知名科学家建立联系。

Intellectual merit. The goal of this Keystone Symposium on "AAA+ and Related ATP-Driven Protein Machines: Structure, Function and Mechanism" is to stimulate the development of novel concepts and approaches directed at a deep understanding of the molecular mechanisms underlying a set of complex cellular processes. The conference focuses on processes that require mechanical work powered by the AAA+ (ATPases Associated with diverse cellular Activities) family of molecular machines. These protein assemblies belong to a special class of enzymes that are involved in key biological processes including transcription of RNA from DNA, the replication of DNA preceding cell division, the biosynthesis and turnover of proteins, the highly regulated processes associated with trafficking of membranes from one part of the cell to another, and remodeling of the dynamic protein scaffolding or "cytoskeleton," which gives the cell its shape and mobility. This meeting will bring together researchers with very diverse backgrounds and perspectives: physical scientists, who are applying biophysical approaches to complex systems, and biologists, who seek deep mechanistic understanding of fundamental cellular processes. This meeting aims to foster innovation by bridging methodological and disciplinary boundaries. Indeed, the meeting program will bring together speakers from diverse fields who typically have had few opportunities to interact but have elucidated critical aspects of different AAA+ proteins. Speakers will also include those who have made breakthroughs in the understanding of proteins that do not belong to the AAA+ family but are either functionally or structurally related. The perspectives transcend both molecular scales, from the cellular to the atomic level, as well as methodologies, from cell biology and genetics to single-molecule biophysics. This combination will likely lead to insights into the common mechanism of these proteins and to novel experimental approaches and discoveries. Broader impacts. This conference was conceived and organized around the central goal to stimulate new ideas and novel approaches in AAA+ protein research. To attain these goals, speakers were selected who have been pioneers in many different biological areas or who have achieved recent conceptual or experimental breakthroughs at different levels of analysis (e.g., structural, chemical, cellular). This meeting will enhance infrastructure by fostering networking and collaborations. With regard to the educational benefits to trainees and new invesitigators in attendance, the meeting will achieve the following: 1) acquaint these individuals with state-of-the-art information; 2) encourage critical scientific analysis; and 3) promote their career development with opportunities to participate in the discussion of cutting-edge science and to network with established scientists who might contribute to their scientific and professional development.