UniformMu: A Transposon Resource for Functional Genomics of Maize

Pi：Donald R McCarty(佛罗里达大学盖恩斯维尔分校)Copi：Karen E.Koch(佛罗里达大学盖恩斯维尔分校)合作者：Carolyn Lawrence(爱荷华州立大学/USDA-ARS)和Marty Sachs(伊利诺伊大学香槟分校/USDA-ARS)玉米功能基因组学公共资源将通过对玉米基因组中数千个转座子插入的测序和图谱来构建。通过在基因中产生突变，转座子为剖析玉米基因的功能提供了一个重要工具。该资源将基于UniformMu人口。UniformMu是一个大型自交系玉米群体，专门为玉米基因组的转座子突变而培育。利用下一代DNA测序技术，将在玉米基因组中定位数千个独特的、可遗传的转座子插入群体。精确映射的插入片段将可以使用MaizeGDB.org基因组浏览器和数据库工具在线搜索。插入链接到高质量、可持续的种子库存，由玉米遗传合作库存中心(maizecoop.cropsor.uiuc.edu)免费向公众分发。通过实现以下目标，该项目将使UniformMu资源的规模和基因组覆盖率大约翻一番：1)为玉米遗传合作库存中心创建5760个富含突变的UniformMu F3系的可持续种子库；2)使用下一代测序方法绘制F3系中独特的生发转座子插入图；以及3)通过MaizeGDB和玉米库存中心提高公众对UniformMu资源的认识和获取。广泛的影响：UniformMu资源的广泛相关性建立在1)玉米对基础和应用生物学研究的重要性；2)UniformMu资源作为玉米功能基因组学工具在美国国内外的独特性；3)研究界预期使用的广度，包括从事其他植物物种研究的科学家；以及4)通过建立的社区和国家资源中心(MaizeGDB、玉米遗传合作库存中心和基因库)传播资源，确保UniformMu资源的长期公众可获得性和可持续性。此外，该项目提供了丰富的培训环境，让不同的本科生和博士后导师参与大规模田间遗传学、转座子生物学、下一代测序技术、生物信息学和基因组学方法的整合。该资源可在MaizeGDB.org上在线获取，种子库存由玉米遗传合作库存中心(maizecoop.cropsor.uiuc.edu)分发。用于定位转座子插入的一致序列保存在Genbank(www.ncbi.nlm.nih.gov)中。

PI: Donald R McCarty (University of Florida - Gainesville)CoPI: Karen E. Koch (University of Florida - Gainesville)Collaborators: Carolyn Lawrence (Iowa State University/USDA-ARS) and Marty Sachs (University of Illinois at Urbana-Champaign/USDA-ARS) A public resource for functional genomics of maize will be constructed by sequencing and mapping of thousands of transposon insertions in the maize genome. By generating mutations in genes, transposons provide an important tool for dissecting the functions of maize genes. The resource will be based on the UniformMu population. UniformMu is a large inbred maize population developed specifically for transposon mutagenesis of the maize genome. Thousands of unique, heritable transposon insertions in the population will be mapped in the maize genome using Next Generation DNA sequencing technology. The precisely mapped insertions will be searchable online using the MaizeGDB.org genome browser and database tools. Insertions are linked online to high-quality, sustainable seed stocks distributed to the public without charge by the Maize Genetics Cooperation Stock Center (maizecoop.cropsci.uiuc.edu). By achieving the following objectives the project will approximately double the size and genome coverage of the UniformMu resource:1) Create sustainable seed stocks of 5,760 mutant enriched UniformMu F3 lines for the Maize Genetics Cooperation Stock Center;2) Map the unique germinal transposon insertions in the F3 lines using Next Generation sequencing methods; and, 3) Enhance public awareness and accessibility of the UniformMu resource via MaizeGDB and the Maize Stock Center.Broader impacts: The broad relevance of the UniformMu resource is founded on 1) the importance of maize to basic and applied biological research in the US; 2) the uniqueness of the UniformMu resource inside and outside the US as a tool for functional genomics of maize; 3) the breadth of expected usage by the research community including scientists working with other plant species; and, 4) the dissemination of the resource through established community and national resource centers (MaizeGDB, Maize Genetics Cooperation Stock Center and Genbank) ensuring long-term public accessibility and sustainability of the UniformMu resource. In addition, the project provides a rich training environment that engages diverse undergraduates and postdoctoral mentors in integration of large scale field genetics, transposon biology, next generation sequencing technology, bioinformatics and genomics methods. The resource is accessible online at MaizeGDB.org and seed stocks are distributed by the Maize Genetics Cooperation Stock Center (maizecoop.cropsci.uiuc.edu). Consensus sequences used to map the transposon insertions are deposited in Genbank (www.ncbi.nlm.nih.gov).