MRI: Ultracentrifuge acquisition to support Bioengineering Red Fluorescent Tag for in vivo Microscopy

本提案所要求的超浓缩设备是研究、表征和生物工程蓝细菌光敏蛋白质的必要设备。有必要从用于生物工程蛋白质的大肠杆菌表达系统中分离蛋白质。Spiller实验室的重点是设计一种用于体内显微镜的小型亮红色荧光蛋白标签。米尔斯学院的本科生、学士后和预科生都从事实验室工作。他们获得研究经验和专业知识，同时参与前沿研究。Spiller实验室的另一个目标是鼓励年轻女性追求科学事业。Spiller实验室于2010年4月获得了NSF-CBET-0967965资助，以资助这项研究。超级电容器是这个项目的关键设备之一。Spiller实验室目前正在与加州大学戴维斯分校生物光子科学与技术中心（CBST）合作开展一项前沿研究项目。他们使用PCR和大肠杆菌表达系统从五（5）种II类（蓝色/绿色）蓝藻色素中捕获、表征和纯化GAF结构域。

The ultracentrifuge that is requested with this proposal is an essential piece of equipment for research characterizing and bioengineering light sensitive proteins from cyanobacteria. It is necessary to separate proteins from the E.coli expression system that are used for bioengineering proteins. The focus of The Spiller Lab is to engineer a small, bright red fluorescent protein tag for in vivo microscopy. Undergraduate, post baccalaureate and glide-year students at Mills College are engaged in the laboratory work. They gain research experience and expertise, while participating in leading edge research. Another goal of Spiller Lab is to encourage young women to pursue scientific careers. Spiller Lab was awarded a NSF-CBET-0967965 grant in April 2010 to fund this research. An ultracentrifuge is one of the critical pieces of equipment for this project. The Spiller Lab is currently working on a leading-edge research project in collaboration with Center for Biophotonics Science and Technology (CBST), University of California, Davis. They are using PCR, and E.coli expression system, to capture, characterize and purify GAF domains from the five (5) class II (blue/green) cyanobacteriochromes.