Comparative Biogeography of the Lesser Sunda Islands of Indonesia: Diversification Across a Wallacean Two-Way Filter Barrier

小巽他群岛是一个双弧群岛，西起爪哇岛和巴厘岛，东至新几内亚。这是世界上地质最活跃、构造最复杂的地区之一。小巽他海峡可能是巽他大陆架（马来半岛、婆罗洲、苏门答腊、爪哇和巴厘岛）和萨胡尔大陆架（澳大利亚新几内亚）之间的“垫脚石”，从而形成了一个双向过滤器，使生物体分散在世界上两个伟大的地理区域，亚洲和澳大利亚-巴布亚之间。本研究通过系统发育和地理学方法，沿着基于聚结的种群遗传学方法，调查了该地区的物种形成和地理学。这项研究是比较性的，调查了12种两栖动物和爬行动物物种复合体。该计划的新颖之处在于，利用下一代DNA测序的力量，使用诱饵捕获方法为每个焦点分类群开发了200个核DNA位点。基因座将使用系统发育和人口遗传学方法进行分析，以评估广泛的地理模式在东南亚，多样化的模式thoughout小孙达斯，在血统边界的基因流，并估计在群岛内的物种形成事件的分歧时间。研究结果将为负责保护这一全球保护热点地区特有物种的印度尼西亚机构提供信息。

The Lesser Sunda Archipelago occurs as a double arc of islands extending between Java and Bali in the west and New Guinea in the east. This is one of the most geologically active and tectonically complex regions in the world. The Lesser Sundas may act as "stepping stones" between the Sunda Shelf (Malay Peninsula, Borneo, Sumatra, Java, and Bali) and Sahul Shelf (New Guinea, Australia), thereby forming a two-way filter for organisms dispersing between two of the world's great biogeographical realms, Asia and Australo-Papua. This study investigates speciation and biogeography of this region by applying phylogenetic and biogeographic methodologies, along with coalescent-based population genetic approaches. The study is comparative, investigating 12 amphibian and reptile species complexes.Novel to this program is the development of 200 nuclear DNA loci for each focal taxon using a bait-capture approach that harnesses the power of Next-Gen DNA sequencing. Loci will be analyzed using phylogenetic and population genetic methods to evaluate broad biogeographic patterns across SE Asia, patterns of diversification thoughout the Lesser Sundas, gene flow at lineage boundaries, and to estimate divergence times for speciation events within the archipelago. Results will inform Indonesian agencies tasked with protecting areas of endemism in this Global Conservation hotspot.