The Development of Microelectrode Arrays as Bioanalytical Tools

1262176 Moeller微电极阵列是监测小分子文库与生物受体相互作用的潜在有力工具。与目前最先进的方法相比，它们有两个主要优势。首先，它们可以用来在结合事件发生时检测它们，而不需要标记的受体或免疫分析。其结果是一种具有成本效益的快速分析方法，使生物数据能够交互地指导新受体配体的设计和合成。其次，阵列中的微电极可用于从电极表面回收分子。因此，文库中产生生物信号的分子可以被分离和表征。这允许对图书馆进行质量控制。考虑到这些进展，已经开发了用于在微电极阵列表面选择性放置、操纵和监测分子的新化学。在这个项目中，我们将通过开发直接合成分子库和覆盖电极的多孔膜来进一步建立工具库，以最大限度地减少非特异性结合，并将展示用于筛选小分子的新型电极阵列的概念验证。一般公开声明：该项目将开发基于电极阵列的技术，以创建和测试候选药物分子。这项技术很有趣，因为它可以用于从生物分子合成到释放到筛选生物分子与目标相互作用的所有阶段。该项目由ENG董事会CBET部门的纳米生物传感计划资助，联合资金来自MPS董事会CHE部门的化学测量和成像(CMI)计划和生命过程化学(CLP)计划。

1262176MoellerMicroelectrode arrays are potentially powerful tools for monitoring interactions between small molecule libraries and biological receptors. They hold two main advantages over current state-of-the-art methods. First, they can be used to detect binding events as they occur without the need for a labeled receptor or immunological assay. The result is a cost effective, rapid method for analysis that enables the use of biological data to interactively guide the design and synthesis of new receptor ligands. Second, the microelectrodes in an array can be used to reclaim molecules from the surface of the electrodes. Hence, molecules in a library that give rise to a biological signal can be isolated and characterized. This allows for quality control of the library. With these advances in mind, new chemistry for the site-selective placement, manipulation, and monitoring of molecules on the surface of a microelectrode array have been developed. In this project we will further build up the repertoire of tools by developing chemistry for direct synthesis of molecular libraries, porous membranes that will cover electrodes to minimize nonspecific binding and will demonstrate proof of concept use of novel electrode arrays for screening small molecules.General public statement:This project will develop technologies based on electrode arrays to enable creation and testing of drug candidate molecules. This technology is interesting in that it can be used at all stages from biomolecule synthesis to its release to screening of biomolecule-target interactions.This project is being funded by the Nano-Biosensing Program in the CBET Division of the ENG directorate, with co-funding from both the Chemical Measurement and Imaging (CMI) Program and the Chemistry of Life Processes (CLP) Program in the CHE Division of the MPS directorate.