MRI: Acquisition of genome sequencers for Bigelow Laboratory for Ocean Sciences

投资促进机构要求提供资金，用于购置环境微生物基因组测序所需的综合仪器系统。这包括DNA提取和测序库准备的测序仪和辅助仪器。所要求的测序仪器将补充毕格罗现有的测序前和测序后分析基础设施，为微生物基因组研究提供全面支持。这项收购将加速现有和新的研究项目，并使新技术开发成为可能。通过向外部用户提供改进和扩展的单细胞基因组学中心(SCGC)服务，它还将使全球研究界受益。广泛影响：拟议仪器的好处将以前所未有的速度扩大我们对海洋浮游生物基因组学的理解。与Bigelow实验室的SCGC的联系非常紧密；在从细菌、古生物和小型真核生物中分离和放大高质量单细胞基因组方面的领先研究使该设施在该领域处于世界领先地位。社会效益与该中心将使用获得的仪器进行的科学的进步有关，并通过一个强大的外展计划，该计划涉及一系列不同倡议的课程，包括本科生研究培训(REU)、科尔比学院学生的学习学期、与缅因州的研讨会，以及为对单细胞基因组学感兴趣的科学家举办的专门研讨会。获得的仪器将用于非常难得的学生和专业级别的动手培训，其中研讨会可以包括从DNA提取到计算分析的从头到尾的体验。

The PIs request funding for the acquisition of an integrated system of instruments required for genomic sequencing of environmental microorganisms. This includes sequencers and supporting instruments for DNA extraction and sequencing library preparation. The requested sequencing instrumentation will complement the already existing infrastructure at Bigelow for pre- and post-sequencing analyses, to provide comprehensive support for microbial genomic studies. This acquisition will accelerate existing and new research programs, and enable novel technology development. By enabling improved and expanded Single Cell Genomics Center (SCGC) services to external users, it will also benefit the global research community.Broader Impacts: The benefits of the proposed instrumentation stand to expand our understanding of marine plankton genomics at an unprecedented rate. The association with the SCGC at Bigelow Laboratory is a very strong connection; where leading research in sorting and amplifying high quality single cell genomes from bacteria, archaea and small eukaryotes has taken this facility to the top in the world for this field. The societal benefits are related to the advancement of the science to be conducted at this center using the acquired instrumentation, and through a strong outreach plan that involves curricula of a diverse suite of initiatives including training in research for undergraduates (REU), study semesters for Colby College students, workshops with the state of Maine, and specialized workshops such as have been held for scientists interested in single cell genomics. The acquired instrumentation will be used for hands-on training at student and professional levels that is very hard to find, in which the workshops can include start-to-finish experiences from DNA extraction through computational analysis.