Characterisation and evolution of chromosome complements of carnivorous Lentibulariaceae

世界分布的香豆科具有极大的可塑性，染色体数目可变，高碱基替换率和独特的捕食机制。这个有趣的植物家族的基因组和染色体互补很少被研究。为Genlisea pygmaea。(76 Mbp；n=15)与最小的植物基因组之一，对于棉属(175 Mbp；n=18或19)和对毛棉(1530 Mbp，n=17或18)，基本倍性水平(二倍体与多倍体)，着丝粒结构(单着丝粒与多着丝粒)，关于异染色质、端粒和rDNA的染色体构成以及表观遗传标记的亚核分布将被确定。将建立一个BAC文库，并将其排序为平铺路径，用于通过比较染色体绘制来阐明Genlisea属和潜在的其他Lentibuariaceae物种的核型进化，特别是染色体数量变化的原因。它的目的是找出，是否相似或不同的机制形成香菇科核型，与其他种子植物科所描述的情况相比较。通过绘制和测序BAC池的相互绘制，可以揭示十字花科植物A.thaliana的同源性。

The cosmopolitan Lentibulariaceae family is characterized by extreme plasticity of genome size, variable chromosome numbers, high base substitution rates and unique trapping mechanisms for carnivory. Genomes and chromosome complements of this interesting plant family are only scarcely studied. For Genlisea pygmaea. (76 Mbp; n=15) with one of the smallest plant genomes, for G. margaretae (175 Mbp; n=18 or 19) and for G. hispidula (1530 Mbp, n=17 or 18), the basic ploidy level (diploidy vs. polyploidy), the centromere structure (monocentric vs. polycentric), the chromosomal constitution regarding heterochromatin, telomeres, and rDNA as well as the subnuclear distribution of epigenetic markers will be determined. A BAC library will be established, ordered into a tiling path and used to elucidate karyotype evolution, in particular the reasons of chromosome number alteration, within the genus Genlisea and potentially other Lentibulariaceae species via comparative chromosome painting. It is aimed to find out, whether similar or alternative mechanisms shaped Lentibulariaceae karyotypes, compared to the situation described for other seed plant families. Reciprocal painting with mapped and sequenced BAC pools will reveal the degree of synteny to A. thaliana, a member of the Brassicaceae family.