EAGER: Novel prognostic microfluidic systems for cell separation from whole blood

在拟议的研究中，微流控技术与血液分离原理结合应用，以满足对简化预后设备的迫切需求，以帮助评估疾病进展和监测治疗。该方法的创新之处在于在单个微流体系统（芯片）中使用双重分离技术，并使用单个全血样本分离血细胞和循环内皮细胞。在血液疾病中，如镰状细胞病，这些细胞持有与疾病病理相关的重要信息。在单个芯片上从外周血中分离多种细胞类型代表了微流控技术的进步，具有可靠评估疾病预后的重大潜力。疾病状态的诊断和监测对健康至关重要。工程工具和系统，如微流体，为开发简单的系统提供了无与伦比的机会，这些系统可以被个人用来评估疾病预后和监测治疗。拟议的研究结合了现有的微流体和分离技术，从血液中分离细胞成分，并提供了一种具有预测非常复杂血液疾病能力的设备。分离和分析这些与疾病病理相关的细胞成分，可能会产生预后验证的生物标志物，并增强评估和治疗策略。少数民族在转化研究中的代表性不足，拟议的项目通过与PI领导的生物医学创新和转化学者（BITS）计划的合作来解决这一差距。BITS旨在为未被充分代表的少数民族研究生和博士后提供创新、转化研究和创业方面的教育和指导。

BarabinoCBET - 1229954In the proposed studies microfluidic technologies are uniquely applied in combination with blood separation principles to meet a pressing need for simplified prognostic devices to aid in assessing disease progression and monitoring treatment. The innovation in this approach is the utilization of dual separation techniques within a single microfluidic system (chip) and using a single whole blood sample to isolate blood cells and circulating endothelial cells. In blood disorders, such as sickle cell disease, these cells hold important information related to disease pathology. The separation of multiple cell types from peripheral blood on a single chip represents an advancement in microfluidic technology that has significant potential for reliable assessment of disease prognosis.Diagnosis and monitoring of disease states is critically important to health. Engineering tools and systems, such as microfluidics, provide unparalleled opportunities for the development of simple systems that can be used by individuals to assess disease prognosis and monitor treatment. The proposed studies combine existing microfluidic and separation technologies to separate cellular components from blood and deliver a device with predictive capabilities for a very complex blood disorder. Separation and analysis of these cellular components, which are linked to disease pathology, are likely to lead to prognostically validated biomarkers and enhance strategies for assessment and treatment. Minorities are underrepresented in translational research and the proposed project addresses this gap through partnership with the Biomedical Innovation and Translation Scholars (BITS) program led by the PI. BITS is designed to provide education and mentoring to underrepresented minority graduate students and postdocs on innovation, translational research and entrepreneurship.