Konfokales Live-Imaging Mikroskop mit Spinning Disc

在分子水平上理解细胞行为的动力学越来越需要在受控环境（CO2，温度）中的体内条件下观察一直到单分子水平的相互作用。我们正在申请一个共聚焦显微镜的研究动态细胞和细胞内的过程中活的样品。该仪器必须提供最先进的空间和时间分辨率。鉴于神经元细胞和组织的脆弱性，高灵敏度和尽可能低的光毒性至关重要。必须为极其敏感的干细胞和神经元样品保持恒定的环境，同时在短期和长期观察期间提供稳定的光学器件。一个完全机动化的显微镜平台与自动对焦能力是必要的以下细胞运动和预测的时间和空间。FRET能力应该是初始配置的一部分。多色转基因小鼠工具的使用需要快速和宽波长的共聚焦激发和检测，以及DIC能力，用于在可见光下同时记录。基于对比度的自动对焦也需要DIC。目前，在Charité没有类似的成像站，能够采用与高灵敏度神经细胞和组织培养相兼容的先进活细胞成像技术。

Understanding the dynamics of cell behavior at the molecular level increasingly requires the observation of interactions all the way to the single molecule level under in vivo conditions in a controlled environment (CO2, temperature). We are applying for a confocal microscope for the study of dynamic cellular and intracellular processes in live samples. The instrument must provide state-of-the-art spatial and temporal resolution. High sensitivity with the lowest possible phototoxicity is essential given the fragile nature of neuronal cells and tissue. A constant environment must be maintained for extremely sensitive stem cell and neuronal samples while providing for stable optics during both short and long term observations. A fully motorized microscope platform with autofocus capability is necessary for following cell movements and projections in time and space. FRET capability should be part of the initial configuration. Use of multi-color genetically modified mouse tools requires fast and broad wavelength confocal excitation and detection, together with DIC capability for simultaneous documentation in visible light. DIC is also required for autofocus based on contrast. At present there is no comparable imaging station at the Charité capable of advanced live cell imaging techniques compatible with highly sensitive neuronal cell and tissue culture.