MRI: Acquisition of a High-Resolution Quadrupole Time-of-Flight Tandem Mass Spectrometer for Advancing Research and Education at the University of Maryland College Park

该奖项由主要研究仪器和化学研究仪器项目支持。马里兰大学帕克分校的Peter Nemes教授及其同事Daniel Falvey， Lyle Isaacs， Lisa Taneyhill和Scott Juntti正在获得一台具有电喷雾电离能力的高分辨率高压液相色谱质谱计（hl - hplc - esi - ms）。一般来说，质谱（MS）是用于鉴定和表征复杂样品中嵌入的少量化学物质的关键分析方法之一。在一个典型的实验中，这些成分流入质谱仪，在那里它们被电离成离子，并测量离子的质量。这种高度灵敏的技术可以研究复杂混合物中的分子结构。带有液相色谱仪的仪器可以在混合物到达质谱仪之前将其分离。在电喷雾技术中，对液体施加高电压以产生气溶胶。这个电压对于大分子产生离子是有用的，因为它避免了大分子在电离时分裂的倾向。此次收购加强了大学和地区的研究基础设施。该仪器通过使用这种现代分析技术使不同群体的学生参与研究和研究培训，从而扩大了参与范围。此次收购还为该机构的许多本科生和研究生以及博士后提供了培训机会。在共享质谱设备中测量小生物和有机分子的新能力通过研讨会以及课程现代化和与鲍伊州立大学的合作，对哥伦比亚-马里兰-弗吉尼亚地区的科学家和学生产生了广泛的影响。颁发质谱仪的目的是为了加强各级的研究和教育。它尤其影响胚胎发育的代谢效应和神经系统功能中激素代谢物活性的研究。该仪器还用于肝脏线粒体代谢以及神经嵴和牙基细胞分子机制的研究。此外，质谱为寻找下一代酶动力学同位素效应和研究将蛋白质分子从大细胞结构中分离出来的含缬氨酸蛋白（VCP）的结合物提供了有用的信息。质谱计也被用于为分子客体化合物的高效合成设计提供信息。它还用于研究亲电含氮物种及其与蛋白质和核酸的反应性。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

This award is supported by the Major Research Instrumentation and the Chemistry Research Instrumentation programs. Professor Peter Nemes from the University of Maryland College Park and colleagues Daniel Falvey, Lyle Isaacs, Lisa Taneyhill and Scott Juntti are acquiring a high-resolution, high-pressure liquid chromatograph mass spectrometer with electrospray ionization capabilities (HR-HPLC-ESI-MS). In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex samples. In a typical experiment, the components flow into a mass spectrometer where they are ionized into ions and the ions' masses are measured. This highly sensitive technique allows the structure of molecules in complex mixtures to be studied. An instrument with a liquid chromatograph can separate mixtures of compounds before they reach the mass spectrometer. In the electrospray technique a high voltage is applied to a liquid to create an aerosol. This voltage is useful to produce ions from large molecules by avoiding the propensity of macromolecules to fragment when ionized. The acquisition strengthens the research infrastructure at the University and regional area. The instrument broadens participation by involving diverse groups of students in research and research training using this modern analytical technique. The acquisition also provides training opportunities to many undergraduate and graduate students as well as postdoctoral fellows at this institution. The new capability to measure both small biological and organic molecules in a shared Mass Spectrometry Facility has a broad impact on scientists and students in the District of Columbia-Maryland-Virginia region through workshops as well as curriculum modernization and collaborations with Bowie State University. The award of the mass spectrometer is aimed at enhancing research and education at all levels. It especially impacts studies of metabolic effectors of embryonic development and hormone metabolite activity in nervous system functions. The instrumentation is also used for research on mitochondrial metabolism in the liver as well as molecular mechanisms in neural crest and dental placode cells. In addition, the MS provides information useful for the search of the next generation enzymatic kinetic isotope effects and for studying binders for the valosine-containing protein (VCP) that segregate protein molecules from large cellular structures. The mass spectrometer is also used to inform the design of efficient syntheses for molecular guest compounds. It is also utilized in investigations of electrophilic nitrogen containing species and their reactivity with proteins and nucleic acids.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.