MRI: Acquisition of a Hybrid Quadrupole Orbitrap LC/MS System to Facilitate Trace Quantitation and Complex Mixture Analyses at the University of Alaska Anchorage

该奖项由主要研究仪器、化学研究仪器计划和既定的激励竞争性研究计划(EPSCoR)支持。阿拉斯加大学安克雷奇分校正在获取一种高性能的台式四极系统，该系统结合了四极、离子路径多极和Orbitrap分析器，以及一种液体，以支持Patrick Tomco教授、Jason Burkhead、Aaron Dotson、Colin McGill和其他同事。通常，质谱仪是用于鉴定和表征复杂样品中少量化学物种的关键分析方法之一。带有液体色谱仪的仪器可以在混合物到达质谱计之前将其分离。在四极质谱计中，有四个相互平行的圆柱形棒，它们充当质量分析器(仪器的部件，负责根据样品离子的质量与电荷比(m/z)选择样品离子)。根据施加到棒上的振荡电场中离子轨迹的稳定性，离子在四极杆中被分离。在这台仪器中，这种能力与离子俘获质量分析器耦合，该分析器由外筒状电极和同轴内轴轴状电极组成，该电极捕获围绕主轴的轨道运动中的离子。利用频率信号的傅里叶变换，检测来自俘获离子的图像，并将其转换为质谱图。此次收购加强了大学和地区的研究基础设施。该仪器通过让不同的学生参与到这一现代分析技术中来扩大参与。它还被用于推广活动，涉及阿拉斯加大学费尔班克斯分校(UAF)和阿拉斯加东南大学(UAS)，以及阿拉斯加渔猎和阿拉斯加州立犯罪实验室等机构以及新奥尔良大学的合作者。授予这种质谱仪的目的是促进整个阿拉斯加大学系统各级的研究和教育。这尤其影响到对杀虫剂鱼藤酮异黄酮在阿拉斯加中南部不断变化的气候中的命运的理解。该仪器还用于探索减轻北极和中亚地区的石油泄漏环境损害，并用于代谢组学的研究。此外，它还有助于分析气候变化对北极和北方生态区植物-草食动物动态的影响，以及研究阿拉斯加植物的药用特性。质谱仪还被用来了解冬眠北极松鼠微生物群中的必需氨基酸代谢，使用15氮标记的氮源。这一奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

This award is supported by the Major Research Instrumentation, the Chemistry Research Instrumentation programs and the Established Program to Stimulate Competitive Research (EPSCoR). The University of Alaska-Anchorage is acquiring a high-performance benchtop quadrupole system that combines a quadrupole, an ion routing multipole and an Orbitrap analyzer and a liquid to support Professor Patrick Tomco, Jason Burkhead, Aaron Dotson, Colin McGill, and other colleagues. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex samples. An instrument with a liquid chromatograph can separate mixtures of compounds before they reach the mass spectrometer. In a quadrupole mass spectrometer, there are four cylindrical rods parallel to each other, that serve as the mass analyzer (the component of the instrument responsible for selecting sample ions based on their mass-to-charge ratio (m/z)). Ions are separated in a quadrupole based on the stability of their trajectories in oscillating electric fields that are applied to the rods. In this instrument, this capability is coupled to an ion trap mass analyzer consisting of an outer barrel-like electrode and a coaxial inner spindle-like electrode that traps ions in an orbital motion around the spindle. The image from the trapped ions is detected and converted to a mass spectrum using the Fourier transform of the frequency signal. The acquisition strengthens the research infrastructure at the university and regional area. The instrument broadens participation by involving diverse students with this modern analytical technique. It is also used in outreach activities involving the University of Alaska-Fairbanks (UAF) and the University of Alaska-Southeast (UAS) as well as agencies such as the Alaska Department of Fish & Game and the Alaska State Crime Lab and collaborators from the University of New Orleans.The award of this mass spectrometer is aimed at enhancing research and education at all levels across the University of Alaska system. It especially impacts understanding the fate of the insecticide rotenone isoflavone in the changing climate of southcentral Alaska. The instrumentation is also used for exploring environmental oil spill damage mitigation in the Artic and Sub-artic and for the study of metabolomics. In addition, it contributes to the analysis of the impact of climate change on plant-herbivore dynamics in the Artic and Boreal ecoregions, and the study of medicinal properties of Alaskan botanicals. The mass spectrometer is also used to understand essential amino acid metabolism in the microbiomes of hibernatic Artic ground squirrels using 15-nitrogen-labeled nitrogen sources.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.