Collaborative: RoL: Molecular patterns for redox sensing and signaling in organismic defense

植物细菌性病害每年都会给农作物造成巨大的危害和重大的经济损失。当遇到病原细菌感染时，植物局部(即在感染部位)和系统地激活防御反应。这个项目的目标是更好地了解这些植物防御反应途径，并更好地了解植物如何抵御细菌感染。该项目将用于为历史悠久的黑人学院和大学的学生提供MS、NC和GA的研究经验。这些伙伴关系的关键目标将是与教师建立可持续的长期合作，在STEM研究方面培训本科生，并为相关的职业发展道路建立一个服务不足的学生渠道。将组织关于包括植物合成生物学在内的项目相关主题的在线研讨会、视频讲座和实践讲习班。了解氧化还原信号网络的分子逻辑和组织结构对于提高我们对真核生物应对环境挑战的适应性系统的理解是必要的。一个具有互补专业知识的多学科团队将从事创新的实验和计算方法，以阐明氧化还原调节的信号通路，这些信号通路支撑植物系统免疫中同步和脉动的基因活动。该项目由三个部分组成。首先，高通量转录图谱将识别植物免疫转录组中重复出现的空间和时间模式。第二，先进的氧化还原蛋白质组学将确定蛋白质半胱氨酸的氧化修饰，重点是调节性硫醇。最后，将产生一个理论和模型框架来概念化和分析信号周期性在植物系统免疫中的意义。该项目的成功实施将影响我们对生物系统中信息编码中氧化还原过程的理解，并产生广泛适用于研究蛋白质氧化还原修饰和生物系统振荡动力学的方法和算法。该项目由分子和细胞生物科学部门的系统和合成生物学集群以及综合组织系统部门的植物-生物相互作用计划共同资助。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

The bacterial diseases of plants lead to substantial crop damage and significant economic losses each year. Plants activate defense responses locally (i.e., at the infection site) and systemically when confronted with pathogenic bacterial infections. The goal of this project is to better understand these plant defense response pathways and to better understand how plants defend against bacterial infections in general. This project will be used to provide research experiences to students from Historically Black Colleges and Universities in MS, NC, and GA. These partnerships' critical goal will be to generate sustainable long-term collaborations with faculty, train undergraduate students in STEM research, and build a pipeline of underserved students for related career tracks. Online symposia, video lectures and practical workshops will be organized on project-related themes including plant synthetic biology. Knowledge of the molecular logic and organization of redox signaling networks is necessary to enhance our understanding of the adaptive system that governs responses to environmental challenges in eukaryotes. A multi-disciplinary team with complementary expertise will engage in innovative experimental and computational approaches to elucidate redox modulated signaling pathways that underpin synchronous and pulsatile gene activity in plant systemic immunity. The project consists of three components. First, high-throughput transcription profiling will identify recurrent spatial and temporal patterns in the plant immune transcriptome. Second, advanced redox proteomics will identify oxidative modifications of protein cysteines with a focus on regulatory thiols. Finally, a theoretical and modeling framework will be generated to conceptualize and analyze the significance of signal periodicity in plant systemic immunity. Successful implementation of the project will impact our understanding of redox processes in information encoding in biological systems and generate methods and algorithms widely applicable to study redox modifications of proteins and oscillatory dynamics of biological systems.This project is co-funded by the Systems and Synthetic Biology cluster in the Division of Molecular and Cellular Biosciences and the Plant-Biotic Interactions Program in the Division of Integrative Organismal Systems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.