Synthesis of Stabilized Origami Structures

该项目的重点是合成小的，共价锁定的折纸结构。 它建立在现代DNA合成的基础上，以生成完全合成的蛋白质复合物大小的设计功能实体，因此可以充分控制一级结构，包括许多异生物质官能团。 设计的纳米结构可以很容易地由DNA形成。 传统的DNA纳米结构仅限于天然核苷酸的官能团，并且在热和机械方面都是不稳定的。 通过杂交组装后引入的共价锁将用于解决这些限制。 重点将放在基于M1.3作为支架链的折纸结构上，长度为704个核苷酸。 这种支架将由合成的寡核苷酸制成。 基于在链组装期间并入的反应性核苷酸，将合成可用于交联的钉合链。 因此，单分子，单分散和热稳定的单位将产生设计的纳米级三维结构。 这包括一个圆柱形的折纸结构，它有一个空腔，以后可能会被加工成一个活性位点。 稳定的折纸应该是可以以单分散形式合成的最大有机分子之一。 它们可以在分子存储、催化或诊断领域中找到应用。

The project proposed focuses on the synthesis of small, covalently locked origami structures. It builds on the power of modern DNA synthesis to generate designed functional entities of the size of protein complexes that are entirely synthetic and are thus prepared with ample control over the primary structure, including many xenobiotic functional groups. Designed nanostructures can readily be formed from DNA. Conventional DNA naostructures are limited to the functional groups of natural nucleotides and are labile, both thermally and mechanically. Covalent locks, introduced after assembly via hybridization, will be used to tackle these limitations. The focus will be on origami structures based on M1.3 as scaffold strand, 704 nucleotides in length. This scaffold will be elaborated from synthetic oligonucleotides. Staple strands will be synthesized that can be used for cross-linking, based on reactive nucleotides, incorporated during chain assembly. Thus, unimolecular, monodisperse and thermally stabilized units will be generated that have designed nanoscale three-dimensional structures. This includes a cylindrical origami structure with a cavity that may later be elaborated into an active site. Stabilized origami should be among the largest organic molecules that can be synthesized in monodisperse form. They may find application in the fields of molecular storage, catalysis, or diagnostics.