Nonlinear Surface Spectroscopic Investigations into the Molecular Mechanism Governing Cell Membrane Repair

安德鲁·P·卡彭特被授予21财年数学和物理科学提升博士后研究奖学金(NSF MPS-Ascend)奖学金，以开展与俄勒冈州立大学化学专业代表不足的群体扩大参与相关的研究和活动。该项目由安德鲁·卡彭特执行，题为《管理细胞膜修复的分子机制的非线性表面光谱调查》，将在指导科学家乔·拜奥的指导下进行。这个项目得到了多学科活动办公室和数学和物理科学化学司的支持，并得到了生物科学局分子和细胞生物科学司分子生物物理计划的支持。膜结合蛋白执行许多细胞功能，包括修复细胞质膜上的撕裂。这一过程还不是很清楚，这个项目将利用振动和频率产生(VSF)光谱来研究特定蛋白质disferlin调控膜修复的机制，包括确定derferlin结合在膜表面的磷脂来启动膜修复过程，阐明dyferlin结合所需的膜填充条件，使用VSF散射光谱直接询问小泡表面的dyferlin，以及建立从多结构域蛋白质的VSF光谱中提取结构信息所需的实验和计算方法。与俄勒冈州立大学的合作将支持扩大美洲原住民在科学、技术、数学和工程领域的参与，俄勒冈州立大学专注于为当地Chemawa印第安人学校的学生和教师提供研究机会和有针对性的STEM外展活动。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

Andrew P. Carpenter is awarded a FY21 Mathematical and Physical Sciences Ascending Postdoctoral Research (NSF MPS-Ascend) Fellowship to conduct a program of research and activities related to broadening participation by groups underrepresented in Chemistry at Oregon State University. The project being performed by Andrew Carpenter, entitled Nonlinear Surface Spectroscopic Investigations into the Molecular Mechanism Governing Cell Membrane Repair, is to be conducted under the guidance of mentoring scientist Joe Baio. This project is supported by the Office of Multidisciplinary Activities and the Chemistry Division in the Mathematical and Physical Sciences, and by the Molecular Biophysics Program of the Molecular and Cellular Biosciences Division of the Biological Sciences Directorate.Membrane-bound proteins carry out many cellular functions, including repair of tears in the cellular plasma membrane. This process is not well understood, and this project will utilize vibrational sum-frequency generation (VSF) spectroscopy to investigate the mechanism governing membrane repair by the specific protein dysferlin, including determining the phospholipids that dysferlin binds at membrane surfaces to initiate the membrane repair process, elucidating the membrane packing conditions necessary for dysferlin binding, directly interrogating dysferlin at vesicle surfaces using VSF scattering spectroscopy, and establishing experimental and computational methodology needed to extract structural information from VSF spectra of multi-domain proteins. Broadening participation of Native Americans in science, technology, math and engineering will be supported by partnering with efforts at Oregon State University that focus on providing research opportunities and targeted STEM outreach events for students and teachers from the local Chemawa Indian school.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.